Primenenie élektroblottinga v kachestve metoda polucheniia obraztsov belkov i ikh fragmentov dliia mikrosekvenirovaniia.

Translated title of the contribution: Use of electroblotting as a method for preparing samples of proteins and their fragments for microsequencing

N. B. Levina, V. Z. Slepak, O. G. Kiselev, V. V. Shemiakin, A. A. Khokhlachev

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Gel electrophoresis in the presence of sodium dodecyl sulphate followed by electroblotting was employed in sample preparation for microsequencing proteins and protein fragments. Three types of solid supports were compared: glass fiber filters modified by aminopropyltriethoxysilane or covered with polybrene, and polyvinylidenedifluoride membranes. N-Terminal amino acid sequences of several proteins (Mr 14-140 kDA were determined on a gas-phase sequencer with the standard programme; 20-200 pmoles of the protein can be assayed by this method.

Translated title of the contributionUse of electroblotting as a method for preparing samples of proteins and their fragments for microsequencing
Original languageRussian
Pages (from-to)24-31
Number of pages8
JournalBioorganicheskaia khimiia
Volume15
Issue number1
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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