Use of a rapid HPLC assay for determination of pharmacokinetic parameters of ibuprofen in patients with cystic fibrosis

Nader Rifai, Masayuki Sakamoto, Terence Law, Vartouhi Galpchian, Neil Harris, Andrew A. Colin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

High doses of ibuprofen have been shown to delay the progression of lung disease without serious adverse effects in patients with cystic fibrosis. To be effective, peak ibuprofen concentration of 50 to 100 mg/L has to be achieved. We developed an HPLC assay to rapidly determine plasma ibuprofen concentration. We used this assay to determine the pharmacokinetics of ibuprofen in patients with cystic fibrosis. The assay possessed linearity up to 500 mg/L, sensitivity to 1 mg/L, average recovery of 98%, and run-to-run precision (n = 23) of 3%. Furthermore, the assay proved to be free of interference from 51 medications. Observed time to peak concentration varied significantly between those receiving ibuprofen tablets (mean + SD, 94 ± 29 min, n = 16) and syrup (30 ± 0 min, n = 4) (P <0.0001). We conclude that the method described here is ideal for therapeutic monitoring of ibuprofen.

Original languageEnglish (US)
Pages (from-to)1812-1816
Number of pages5
JournalClinical chemistry
Volume42
Issue number11
StatePublished - Nov 14 1996

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Keywords

  • drug monitoring
  • nonsteroidal antiinflammatory drugs

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Rifai, N., Sakamoto, M., Law, T., Galpchian, V., Harris, N., & Colin, A. A. (1996). Use of a rapid HPLC assay for determination of pharmacokinetic parameters of ibuprofen in patients with cystic fibrosis. Clinical chemistry, 42(11), 1812-1816.