Use of a novel spectral bio-imaging system as an imaging oximeter in intact rat brain

Dirk G. Soenksen, Thomas Sick, Yuval Garini

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The use of reflection spectrophotometry to measure the spectra of oxy-hemoglobin and deoxy-hemoglobin, strong absorbers of light in the visible region of the spectrum, is a well established method for determining tissue oxygenation. This type of spectral measurement is typically made with a point-spectrometer and provides information only at a single point. An imaging spectrometer, on the other, can measure the hemoglobin spectra at every pixel in the image, thus providing a two-dimensional (spatial) map of tissue ischemia. A novel spectral bio-imaging system based on the SpectraCube™ technology, an optical method based on proven Fourier transform (FT) spectroscopy, has been applied successfully in intact rat brain to measure oxy- and deoxy-hemoglobin spectra. Spectral images containing 10,000 spectra were acquired in a rat ventilated with 30% O 2, and repeated when the inspired gas mixture was switched for 45 seconds to 100% nitrogen. Differences in hemoglobin spectra corresponding to real differences in tissue oxygenation are readily apparent under these two conditions. There is also some evidence that information concerning cytochromes is present in these spectral images, and algorithms are currently being developed to extract the signatures of cytochromes. Details of the spectral bio-imaging system and the results of the measurements made in intact rat brain are discussed.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Pages182-189
Number of pages8
Volume2679
DOIs
StatePublished - 1996
EventAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy - San Jose, CA, United States
Duration: Jan 29 1996Jan 29 1996

Other

OtherAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy
CountryUnited States
CitySan Jose, CA
Period1/29/961/29/96

Fingerprint

Oximeters
Hemoglobin
Imaging System
Imaging systems
rats
brain
Rats
Brain
Hemoglobins
Imaging
hemoglobin
Imaging techniques
oxyhemoglobin
Oxygenation
oxygenation
cytochromes
Cytochromes
Tissue
Spectrometers
Proteins

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Soenksen, D. G., Sick, T., & Garini, Y. (1996). Use of a novel spectral bio-imaging system as an imaging oximeter in intact rat brain. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2679, pp. 182-189) https://doi.org/10.1117/12.237587

Use of a novel spectral bio-imaging system as an imaging oximeter in intact rat brain. / Soenksen, Dirk G.; Sick, Thomas; Garini, Yuval.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2679 1996. p. 182-189.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Soenksen, DG, Sick, T & Garini, Y 1996, Use of a novel spectral bio-imaging system as an imaging oximeter in intact rat brain. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 2679, pp. 182-189, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases III: Optical Biopsy, San Jose, CA, United States, 1/29/96. https://doi.org/10.1117/12.237587
Soenksen DG, Sick T, Garini Y. Use of a novel spectral bio-imaging system as an imaging oximeter in intact rat brain. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2679. 1996. p. 182-189 https://doi.org/10.1117/12.237587
Soenksen, Dirk G. ; Sick, Thomas ; Garini, Yuval. / Use of a novel spectral bio-imaging system as an imaging oximeter in intact rat brain. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2679 1996. pp. 182-189
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