Urothelial cell culture

Stratified urothelial sheet and three-dimensional growth of urothelial structure

Yuanyuan Zhang, Anthony Atala

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Citations (Scopus)

Abstract

Urothelial cells line the urinary tract, including the renal pelvis, ureters, bladder, superior urethra, and the central ducts of the prostate. They are highly specialized epithelial cell types possessing unique features, imparting important functional roles in the urinary system. They act as a permeability barrier and protect underlying muscle tissues from the caustic effects of urine while also expanding with bladder filling to adjust urine pressures. The multilayered urothelium is typically structured with differentiated, mature surface cells and less mature basal cells. The basal cell layer contains tissue-specific stem cells able to self-renew for the lifetime of the mammal and also produces a pool of maturing cells for tissue homeostasis. Maintaining regenerative basal cells in a culture facilitates urothelial cell growth in vitro. Additionally, epithelial-mesenchymal communication, epithelial-matrix interactions, and cytokines/growth factors are required to maintain the normal structure and function of mature urothelial cells in vitro and to induce stem cell differentiation into urothelial cells. These cultures are useful to study the biology and physiology of the urinary tract, particularly for the development of cell-based tissue engineering strategies in urology. This chapter describes methods for the isolation of urothelial cells and their maintenance in monolayer culture, and methods for the production of multilayer urothelial cell sheets and three-dimensional cocultures of urothelial and mesenchymal cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages383-399
Number of pages17
Volume945
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume945
ISSN (Print)10643745

Fingerprint

Cell Culture Techniques
Growth
Urinary Tract Physiological Phenomena
Urinary Bladder
Stem Cells
Urine
Urothelium
Caustics
Kidney Pelvis
Cell Separation
Urology
Urethra
Tissue Engineering
Ureter
Coculture Techniques
Urinary Tract
Prostate
Cell Differentiation
Mammals
Permeability

Keywords

  • Bladder
  • Epidermal growth factor
  • Scaffold
  • Serum-free medium
  • Tissue engineering
  • Ureter
  • Urinary reconstruction
  • Urothelial cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zhang, Y., & Atala, A. (2013). Urothelial cell culture: Stratified urothelial sheet and three-dimensional growth of urothelial structure. In Methods in Molecular Biology (Vol. 945, pp. 383-399). (Methods in Molecular Biology; Vol. 945). https://doi.org/10.1007/978-1-62703-125-7_23

Urothelial cell culture : Stratified urothelial sheet and three-dimensional growth of urothelial structure. / Zhang, Yuanyuan; Atala, Anthony.

Methods in Molecular Biology. Vol. 945 2013. p. 383-399 (Methods in Molecular Biology; Vol. 945).

Research output: Chapter in Book/Report/Conference proceedingChapter

Zhang, Y & Atala, A 2013, Urothelial cell culture: Stratified urothelial sheet and three-dimensional growth of urothelial structure. in Methods in Molecular Biology. vol. 945, Methods in Molecular Biology, vol. 945, pp. 383-399. https://doi.org/10.1007/978-1-62703-125-7_23
Zhang Y, Atala A. Urothelial cell culture: Stratified urothelial sheet and three-dimensional growth of urothelial structure. In Methods in Molecular Biology. Vol. 945. 2013. p. 383-399. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-125-7_23
Zhang, Yuanyuan ; Atala, Anthony. / Urothelial cell culture : Stratified urothelial sheet and three-dimensional growth of urothelial structure. Methods in Molecular Biology. Vol. 945 2013. pp. 383-399 (Methods in Molecular Biology).
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