Abstract
Urothelial cells line the urinary tract, including the renal pelvis, ureters, bladder, superior urethra, and the central ducts of the prostate. They are highly specialized epithelial cell types possessing unique features, imparting important functional roles in the urinary system. They act as a permeability barrier and protect underlying muscle tissues from the caustic effects of urine while also expanding with bladder filling to adjust urine pressures. The multilayered urothelium is typically structured with differentiated, mature surface cells and less mature basal cells. The basal cell layer contains tissue-specific stem cells able to self-renew for the lifetime of the mammal and also produces a pool of maturing cells for tissue homeostasis. Maintaining regenerative basal cells in a culture facilitates urothelial cell growth in vitro. Additionally, epithelial-mesenchymal communication, epithelial-matrix interactions, and cytokines/growth factors are required to maintain the normal structure and function of mature urothelial cells in vitro and to induce stem cell differentiation into urothelial cells. These cultures are useful to study the biology and physiology of the urinary tract, particularly for the development of cell-based tissue engineering strategies in urology. This chapter describes methods for the isolation of urothelial cells and their maintenance in monolayer culture, and methods for the production of multilayer urothelial cell sheets and three-dimensional cocultures of urothelial and mesenchymal cells.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 383-399 |
| Number of pages | 17 |
| Volume | 945 |
| DOIs | |
| State | Published - Jan 1 2013 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 945 |
| ISSN (Print) | 10643745 |
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Keywords
- Bladder
- Epidermal growth factor
- Scaffold
- Serum-free medium
- Tissue engineering
- Ureter
- Urinary reconstruction
- Urothelial cells
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Urothelial cell culture : Stratified urothelial sheet and three-dimensional growth of urothelial structure. / Zhang, Yuanyuan; Atala, Anthony.
Methods in Molecular Biology. Vol. 945 2013. p. 383-399 (Methods in Molecular Biology; Vol. 945).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Urothelial cell culture
T2 - Stratified urothelial sheet and three-dimensional growth of urothelial structure
AU - Zhang, Yuanyuan
AU - Atala, Anthony
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Urothelial cells line the urinary tract, including the renal pelvis, ureters, bladder, superior urethra, and the central ducts of the prostate. They are highly specialized epithelial cell types possessing unique features, imparting important functional roles in the urinary system. They act as a permeability barrier and protect underlying muscle tissues from the caustic effects of urine while also expanding with bladder filling to adjust urine pressures. The multilayered urothelium is typically structured with differentiated, mature surface cells and less mature basal cells. The basal cell layer contains tissue-specific stem cells able to self-renew for the lifetime of the mammal and also produces a pool of maturing cells for tissue homeostasis. Maintaining regenerative basal cells in a culture facilitates urothelial cell growth in vitro. Additionally, epithelial-mesenchymal communication, epithelial-matrix interactions, and cytokines/growth factors are required to maintain the normal structure and function of mature urothelial cells in vitro and to induce stem cell differentiation into urothelial cells. These cultures are useful to study the biology and physiology of the urinary tract, particularly for the development of cell-based tissue engineering strategies in urology. This chapter describes methods for the isolation of urothelial cells and their maintenance in monolayer culture, and methods for the production of multilayer urothelial cell sheets and three-dimensional cocultures of urothelial and mesenchymal cells.
AB - Urothelial cells line the urinary tract, including the renal pelvis, ureters, bladder, superior urethra, and the central ducts of the prostate. They are highly specialized epithelial cell types possessing unique features, imparting important functional roles in the urinary system. They act as a permeability barrier and protect underlying muscle tissues from the caustic effects of urine while also expanding with bladder filling to adjust urine pressures. The multilayered urothelium is typically structured with differentiated, mature surface cells and less mature basal cells. The basal cell layer contains tissue-specific stem cells able to self-renew for the lifetime of the mammal and also produces a pool of maturing cells for tissue homeostasis. Maintaining regenerative basal cells in a culture facilitates urothelial cell growth in vitro. Additionally, epithelial-mesenchymal communication, epithelial-matrix interactions, and cytokines/growth factors are required to maintain the normal structure and function of mature urothelial cells in vitro and to induce stem cell differentiation into urothelial cells. These cultures are useful to study the biology and physiology of the urinary tract, particularly for the development of cell-based tissue engineering strategies in urology. This chapter describes methods for the isolation of urothelial cells and their maintenance in monolayer culture, and methods for the production of multilayer urothelial cell sheets and three-dimensional cocultures of urothelial and mesenchymal cells.
KW - Bladder
KW - Epidermal growth factor
KW - Scaffold
KW - Serum-free medium
KW - Tissue engineering
KW - Ureter
KW - Urinary reconstruction
KW - Urothelial cells
UR - http://www.scopus.com/inward/record.url?scp=84870542356&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870542356&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-125-7_23
DO - 10.1007/978-1-62703-125-7_23
M3 - Chapter
SN - 9781627031240
VL - 945
T3 - Methods in Molecular Biology
SP - 383
EP - 399
BT - Methods in Molecular Biology
ER -