Urothelial cell culture

Yuanyuan Zhang, Anthony Atala

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

This chapter reviews the use of urothelial cells as a means to enhance tissue regeneration and wound healing in urinary tract system. It addresses the properties of urothelial cells, including their role as a permeability barrier to protect underlying muscle tissue from the caustic effects of urine and as one of the main cell types, along with smooth muscle cells, that are used in urethral or bladder tissue engineering today. This description includes a general overview of various isolation techniques and culture methods that have been developed to improve urinary tract reconstruction in vivo and aid the characterization of growth factor expression in vitro. The chapter then describes various applications using urothelial cells, including production of multilayer urothelial sheets, tissue engineered bladder mucosa, tissue engineered urethra, and tissue engineered bladder. It also outlines the advantages of sandwich and layered coculture of these cells and the effects of epithelial-stromal cell interactions during tissue regeneration or wound healing processes in the urinary tract.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages27-43
Number of pages17
Volume1037
ISBN (Print)9781627035040
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1037
ISSN (Print)10643745

Fingerprint

Cell Culture Techniques
Urinary Tract
Urinary Bladder
Wound Healing
Regeneration
Culture Techniques
Caustics
Urethra
Tissue Engineering
Stromal Cells
Coculture Techniques
Cell Communication
Smooth Muscle Myocytes
Permeability
Intercellular Signaling Peptides and Proteins
Mucous Membrane
Epithelial Cells
Urine
Muscles

Keywords

  • Bladder
  • Epidermal growth factor
  • Scaffold
  • Serum-free medium
  • Tissue engineering
  • Ureter
  • Urinary reconstruction
  • Urothelial cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zhang, Y., & Atala, A. (2013). Urothelial cell culture. In Methods in Molecular Biology (Vol. 1037, pp. 27-43). (Methods in Molecular Biology; Vol. 1037). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-505-7_2

Urothelial cell culture. / Zhang, Yuanyuan; Atala, Anthony.

Methods in Molecular Biology. Vol. 1037 Humana Press Inc., 2013. p. 27-43 (Methods in Molecular Biology; Vol. 1037).

Research output: Chapter in Book/Report/Conference proceedingChapter

Zhang, Y & Atala, A 2013, Urothelial cell culture. in Methods in Molecular Biology. vol. 1037, Methods in Molecular Biology, vol. 1037, Humana Press Inc., pp. 27-43. https://doi.org/10.1007/978-1-62703-505-7_2
Zhang Y, Atala A. Urothelial cell culture. In Methods in Molecular Biology. Vol. 1037. Humana Press Inc. 2013. p. 27-43. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-505-7_2
Zhang, Yuanyuan ; Atala, Anthony. / Urothelial cell culture. Methods in Molecular Biology. Vol. 1037 Humana Press Inc., 2013. pp. 27-43 (Methods in Molecular Biology).
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