Urinary and plasma vitamin D3 metabolites in the nephrotic syndrome

P. W. Lambert, P. B. De Oreo, I. Y. Fu, D. M. Kaetzel, K. von Ahn, B. W. Hollis, B. A. Roos

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Using newly developed and established extraction, Lipidex-5000 chromatography, normal phase gradient HPLC, and ligand binding assay techniques we have directly measured plasma and urine levels of vitamin D3 and its metabolites in seven normal subjects and seven patients with nephrotic syndrome and normal renal function. Significant reductions in the plasma levels of vitamin D,, 24,25(OH)2D3, 25,26(OH)2D,, and 1,25(OH)2D, were noted in all nephrotic patients. In conjunction with the plasma metabolite abnormalities, direct quantitative analysis of the urine in these patients revealed significant increases in nonconjugated 250HD,, 24,25(OH)2D3, and 1,25(OH)2D,. Significant correlations were noted between the plasma and/or urine metabolites and other mineral homeostatic parameters. The results indicate that the primary basis for the reductions in plasma vitamin D, and its metabolites in the nephrotic syndrome is enhanced urinary excretion. The findings of normal serum ionized Ca and i-PTH levels in the patients with nephrosis suggest that reductions in bound and not free forms of vitamin D3 metabolites in plasma may occur in the initial stages of the nephrotic syndrome.

Original languageEnglish (US)
Pages (from-to)7-15
Number of pages9
JournalMetabolic Bone Disease and Related Research
Volume4
Issue number1
DOIs
StatePublished - 1982

Keywords

  • Nephrotic Syndrome
  • Vitamin D Metabolites

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Surgery

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    Lambert, P. W., De Oreo, P. B., Fu, I. Y., Kaetzel, D. M., von Ahn, K., Hollis, B. W., & Roos, B. A. (1982). Urinary and plasma vitamin D3 metabolites in the nephrotic syndrome. Metabolic Bone Disease and Related Research, 4(1), 7-15. https://doi.org/10.1016/0221-8747(82)90003-0