Abstract
Using newly developed and established extraction, Lipidex-5000 chromatography, normal phase gradient HPLC, and ligand binding assay techniques we have directly measured plasma and urine levels of vitamin D3 and its metabolites in seven normal subjects and seven patients with nephrotic syndrome and normal renal function. Significant reductions in the plasma levels of vitamin D,, 24,25(OH)2D3, 25,26(OH)2D,, and 1,25(OH)2D, were noted in all nephrotic patients. In conjunction with the plasma metabolite abnormalities, direct quantitative analysis of the urine in these patients revealed significant increases in nonconjugated 250HD,, 24,25(OH)2D3, and 1,25(OH)2D,. Significant correlations were noted between the plasma and/or urine metabolites and other mineral homeostatic parameters. The results indicate that the primary basis for the reductions in plasma vitamin D, and its metabolites in the nephrotic syndrome is enhanced urinary excretion. The findings of normal serum ionized Ca and i-PTH levels in the patients with nephrosis suggest that reductions in bound and not free forms of vitamin D3 metabolites in plasma may occur in the initial stages of the nephrotic syndrome.
| Original language | English |
|---|---|
| Pages (from-to) | 7-15 |
| Number of pages | 9 |
| Journal | Metabolic Bone Disease and Related Research |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1982 |
| Externally published | Yes |
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Keywords
- Nephrotic Syndrome
- Vitamin D Metabolites
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Surgery
Cite this
Urinary and plasma vitamin D3 metabolites in the nephrotic syndrome. / Lambert, P. W.; De Oreo, P. B.; Fu, I. Y.; Kaetzel, D. M.; von Ahn, K.; Hollis, B. W.; Roos, B. A.
In: Metabolic Bone Disease and Related Research, Vol. 4, No. 1, 01.01.1982, p. 7-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Urinary and plasma vitamin D3 metabolites in the nephrotic syndrome
AU - Lambert, P. W.
AU - De Oreo, P. B.
AU - Fu, I. Y.
AU - Kaetzel, D. M.
AU - von Ahn, K.
AU - Hollis, B. W.
AU - Roos, B. A.
PY - 1982/1/1
Y1 - 1982/1/1
N2 - Using newly developed and established extraction, Lipidex-5000 chromatography, normal phase gradient HPLC, and ligand binding assay techniques we have directly measured plasma and urine levels of vitamin D3 and its metabolites in seven normal subjects and seven patients with nephrotic syndrome and normal renal function. Significant reductions in the plasma levels of vitamin D,, 24,25(OH)2D3, 25,26(OH)2D,, and 1,25(OH)2D, were noted in all nephrotic patients. In conjunction with the plasma metabolite abnormalities, direct quantitative analysis of the urine in these patients revealed significant increases in nonconjugated 250HD,, 24,25(OH)2D3, and 1,25(OH)2D,. Significant correlations were noted between the plasma and/or urine metabolites and other mineral homeostatic parameters. The results indicate that the primary basis for the reductions in plasma vitamin D, and its metabolites in the nephrotic syndrome is enhanced urinary excretion. The findings of normal serum ionized Ca and i-PTH levels in the patients with nephrosis suggest that reductions in bound and not free forms of vitamin D3 metabolites in plasma may occur in the initial stages of the nephrotic syndrome.
AB - Using newly developed and established extraction, Lipidex-5000 chromatography, normal phase gradient HPLC, and ligand binding assay techniques we have directly measured plasma and urine levels of vitamin D3 and its metabolites in seven normal subjects and seven patients with nephrotic syndrome and normal renal function. Significant reductions in the plasma levels of vitamin D,, 24,25(OH)2D3, 25,26(OH)2D,, and 1,25(OH)2D, were noted in all nephrotic patients. In conjunction with the plasma metabolite abnormalities, direct quantitative analysis of the urine in these patients revealed significant increases in nonconjugated 250HD,, 24,25(OH)2D3, and 1,25(OH)2D,. Significant correlations were noted between the plasma and/or urine metabolites and other mineral homeostatic parameters. The results indicate that the primary basis for the reductions in plasma vitamin D, and its metabolites in the nephrotic syndrome is enhanced urinary excretion. The findings of normal serum ionized Ca and i-PTH levels in the patients with nephrosis suggest that reductions in bound and not free forms of vitamin D3 metabolites in plasma may occur in the initial stages of the nephrotic syndrome.
KW - Nephrotic Syndrome
KW - Vitamin D Metabolites
UR - http://www.scopus.com/inward/record.url?scp=0020017922&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020017922&partnerID=8YFLogxK
U2 - 10.1016/0221-8747(82)90003-0
DO - 10.1016/0221-8747(82)90003-0
M3 - Article
C2 - 6289039
AN - SCOPUS:0020017922
VL - 4
SP - 7
EP - 15
JO - Bone
JF - Bone
SN - 8756-3282
IS - 1
ER -