Uptake, Hydrolysis, and Metabolism of Pyridoxine-5′-β -D-Glucoside in Caco-2 Cells

Amy D. Mackey, Robert J. McMahon, Justin Townsend, Jesse F. Gregory

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An important dietary source of vitamin B-6, pyridoxine-5′-β -D-glucoside (PNG), exhibits only partial bioavailability, which is limited by the extent of enzymatic cleavage of the β-glucosidic bond to release metabolically available pyridoxine (PN). This laboratory showed that the intestinal hydrolysis of PNG is catalyzed by cytosolic PNG hydrolase (PNGH) and brush border lactase-phlorizin hydrolase (LPH). LPH-catalyzed PNG hydrolysis in vitro is competitively inhibited by lactose. In the present study, the uptake and hydrolysis of PNG were examined in Caco-2 human colon carcinoma cells, which express a functional LPH but exhibit no PNGH activity. PNG uptake at 37°C was linear over 5-500 μmol/L PNG. Uptake was not significantly reduced when Na+ was substituted with K+, Li+, or Tris in the medium. Increasing PNG concentration in the medium did not change intracellular concentrations of PN, pyridoxamine (PM), pyridoxamine 5′-phosphate (PMP), or pyridoxal 5′-phosphate (PLP); however, intracellular pyridoxal (PL) concentration increased. Intracellular PNG concentration was not significantly reduced in the presence of lactose, but the concentration of PL declined in proportion to extracellular lactose (P = 0.01). These results indicate that PNG can be absorbed intact in a Na +-independent process and is taken up by passive diffusion. The presence of lactose in this in vitro model of intestinal uptake reduced the enzymatic hydrolysis of PNG by lactase.

Original languageEnglish (US)
Pages (from-to)842-846
Number of pages5
JournalJournal of Nutrition
Volume134
Issue number4
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Caco-2 Cells
pyridoxine
glucosides
Hydrolysis
hydrolysis
uptake mechanisms
metabolism
Lactase-Phlorizin Hydrolase
Lactose
cells
Pyridoxal
lactose
Pyridoxine
pyridoxamine
pyridoxal
Pyridoxamine
hydrolases
5'-O-(glucopyranosyl)pyridoxine
Lactase
Vitamin B 6

Keywords

  • Bioavailability
  • Caco-2 cells
  • Lactase-phlorizin hydrolase
  • Pyridoxine-5′-β-D-glucoside (PNG)

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Uptake, Hydrolysis, and Metabolism of Pyridoxine-5′-β -D-Glucoside in Caco-2 Cells. / Mackey, Amy D.; McMahon, Robert J.; Townsend, Justin; Gregory, Jesse F.

In: Journal of Nutrition, Vol. 134, No. 4, 04.2004, p. 842-846.

Research output: Contribution to journalArticle

Mackey, AD, McMahon, RJ, Townsend, J & Gregory, JF 2004, 'Uptake, Hydrolysis, and Metabolism of Pyridoxine-5′-β -D-Glucoside in Caco-2 Cells', Journal of Nutrition, vol. 134, no. 4, pp. 842-846.
Mackey, Amy D. ; McMahon, Robert J. ; Townsend, Justin ; Gregory, Jesse F. / Uptake, Hydrolysis, and Metabolism of Pyridoxine-5′-β -D-Glucoside in Caco-2 Cells. In: Journal of Nutrition. 2004 ; Vol. 134, No. 4. pp. 842-846.
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