Uptake, handling and excretion of Na+ and Cl- from the diet in vivo in freshwater- and seawater-acclimated killifish, Fundulus heteroclitus, an agastric teleost

Carol Bucking, Chris Wood, Martin Grosell

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A radiotracer approach using diets labelled with 22Na+, 36Cl- and [14C]polyethylene-4000 (PEG-4000) was employed to investigate the role of intestinal uptake from the food in ion homeostasis in the killifish Fundulus heteroclitus. This euryhaline teleost lacks both a stomach and the capacity for Cl- uptake at the gills in freshwater. PEG-4000 appearance in the water was minimal up to 10-11?h post-feeding, indicating the virtual absence of Na+ and Cl- loss in the faeces up until this time. Rapid uptake of dietary Na+ and Cl- occurred and more than 88% of 22Na+ and 36Cl- were absorbed in the intestine by 3?h post-feeding; excretion rates of Na+ and Cl- originating from the food were greatest during this period. Uptake and excretion of Cl- from the diet was fivefold to sixfold greater than that of Na+ in freshwater, and approximately threefold greater in seawater. Excretions of dietary Na+ and Cl- by seawater-acclimated killifish were far greater than by freshwater-acclimated killifish in this time frame, reflecting the much greater branchial efflux rates and turnover rates of the internal exchangeable pools. At both 3 and 9?h post-feeding, the largest fraction of dietary Na+ was found in the carcass of freshwater-acclimated fish, followed by the external water, and finally the digestive tract. However, in seawater-acclimated fish, more was excreted to the water, and less was retained in the carcass. For Cl-, which was taken up and excreted more rapidly than Na+, the majority of the dietary load had moved to the external water by 9?h in both freshwater and seawater animals. After 7?days training on a low-salt natural diet (live Lumbriculus variegatus worms; 31.5?μmol?Na+?g-1?wet mass) versus a high-salt synthetic pellet diet (911?μmol?Na+?g-1?dry food mass), freshwater killifish exhibited a lower absolute excretion rate of Na+ from the low-salt diet, but relative uptake from the intestine and retention in the carcass were virtually identical from the two diets. Seawater killifish excreted relatively more Na+ from the low-salt diet. Overall, our results emphasize the importance of dietary Na+ and Cl- in the electrolyte economy of the killifish, particularly in freshwater, and especially for Cl-.

Original languageEnglish (US)
Pages (from-to)3925-3936
Number of pages12
JournalJournal of Experimental Biology
Volume216
Issue number20
DOIs
StatePublished - Oct 1 2013

Keywords

  • Chloride uptake
  • Defaecation
  • Feeding
  • Gills
  • Intestine
  • Ion flux rates
  • Ionoregulation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Physiology
  • Insect Science
  • Aquatic Science

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