Uptake and release of neurotransmitter candidates, [3h]serotonin, [3H]glutamate, and [3H]γ-Aminobutyric acid, in taste buds of the mudpuppy, Necturus maculosus

Takatoshi Nagai, Rona J. Delay, Joan Welton, Stephen D Roper

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Neurotransmitters in vertebrate taste buds have not yet been identified with confidence. Serotonin, glutamate, and γ-aminobutyric acid (GABA) have been postulated, but the evidence is incomplete. We undertook an autoradiographic study of [3H]serotonin, [3H]glutamate, and [3H]GABA uptake in lingual epithelium from the amphibian, Necturus maculosus, to determine whether taste bud cells would accumulate and release these substances. Lingual epithelium containing taste buds was incubated in low concentrations (0.4-6 μM) of these tritiated transmitter candidates and the tissue was processed for light microscopic autoradiography. Merkel-like basal taste cells accumulated [3H]serotonin. When the tissue was treated with 40 mM K+ after incubating the tissue in [3H]serotonin, cells released the radiolabelled transmitter. Furthermore, depolarization (KCl)-induced release of [3H]serotonin was Ca-dependent: if Ca2+ was reduced to 0.4 mM and 20 mM Mg2+ added to the high K+ bathing solution, Merkel-like basal cells did not release [3H]serotonin. In contrast, [3H]glutamate was taken up by several cell types, including non-sensory epithelial cells, Schwann cells, and some taste bud cells. [3H]glutamate was not released by depolarizing the tissue with 40 mM K+. [3H]GABA uptake was also widespread, but did not occur in taste bud cells. [3H]GABA accumulated in non-sensory epithelial cells and Schwann cells. These data support the hypothesis that serotonin is a neurotransmitter or neuromodulator released by Merkel-like basal cells in Necturus taste buds. The data do not support (nor rule out) a neurotransmitter role for glutamate or GABA in taste buds.

Original languageEnglish
Pages (from-to)199-208
Number of pages10
JournalJournal of Comparative Neurology
Volume392
Issue number2
DOIs
StatePublished - Mar 9 1998

Fingerprint

Necturus maculosus
Necturus
Taste Buds
Aminobutyrates
Neurotransmitter Agents
Glutamic Acid
Serotonin
Schwann Cells
Tongue
Epithelium
Epithelial Cells
Amphibians
Autoradiography
Vertebrates

Keywords

  • Excitatory amino acids
  • Gustatory
  • Merkel cells
  • Monoamines
  • Synapses

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Uptake and release of neurotransmitter candidates, [3h]serotonin, [3H]glutamate, and [3H]γ-Aminobutyric acid, in taste buds of the mudpuppy, Necturus maculosus. / Nagai, Takatoshi; Delay, Rona J.; Welton, Joan; Roper, Stephen D.

In: Journal of Comparative Neurology, Vol. 392, No. 2, 09.03.1998, p. 199-208.

Research output: Contribution to journalArticle

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