Upregulation of c-fos protein in rat retina induced by sub-visible threshold diode laser treatment

R. Wen, T. J. Wolfensberge, C. Liu, M. Peng

Research output: Contribution to journalArticlepeer-review


Purpose. This study assesses laser induced biochemical changes, such as the expression of c-fos, an early responsive gene, in the retina after low-energy, subvisible threshold diode laser treatment. Methods. Adult male Long-Evans rats were anesthetized with halothan. Four large (2 mm) spots of laser irradiation were delivered to the retina from a diode laser (810 nm. OcuLight SLx, IRIS Medical Instruments, Inc.) mounted on a dissecting microscope. Retinas were collecfed al various time points after treatmenl. Western blot analysis was performed to determine the protein expression of c-fos. Rgsults. Upregulation of c-fos protein was already detected at an energy level of 0.5 mJ/mnr (2 mm spot size, 50 mW, 30 ms). A! an increased energy level of 1 mJ/mm: (2 mm spot size, 100 mW, 30 ms), induction of c-fos protein was detected as early as 30 min after Ireatment which reached a peak at 4 hr after treatment, and then declined to control level by 8 hr after treatment. No visible change in the retina was observed ophlhalmoscopically during the laser treatment. Conclusions. Our results indicate that expression of c-fos is stimulated in a dynamic fashion by diode laser irradiation at energy levels as low as 0.5-1 mJ/mrrr without inducing visible damage to the retina. Expression of other genes evoked by low energy diode laser irradiation is under investigation.

Original languageEnglish (US)
Pages (from-to)S83
JournalInvestigative Ophthalmology and Visual Science
Issue number4
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Upregulation of c-fos protein in rat retina induced by sub-visible threshold diode laser treatment'. Together they form a unique fingerprint.

Cite this