Purpose. This study assesses laser induced biochemical changes, such as the expression of c-fos, an early responsive gene, in the retina after low-energy, subvisible threshold diode laser treatment. Methods. Adult male Long-Evans rats were anesthetized with halothan. Four large (2 mm) spots of laser irradiation were delivered to the retina from a diode laser (810 nm. OcuLight SLx, IRIS Medical Instruments, Inc.) mounted on a dissecting microscope. Retinas were collecfed al various time points after treatmenl. Western blot analysis was performed to determine the protein expression of c-fos. Rgsults. Upregulation of c-fos protein was already detected at an energy level of 0.5 mJ/mnr (2 mm spot size, 50 mW, 30 ms). A! an increased energy level of 1 mJ/mm: (2 mm spot size, 100 mW, 30 ms), induction of c-fos protein was detected as early as 30 min after Ireatment which reached a peak at 4 hr after treatment, and then declined to control level by 8 hr after treatment. No visible change in the retina was observed ophlhalmoscopically during the laser treatment. Conclusions. Our results indicate that expression of c-fos is stimulated in a dynamic fashion by diode laser irradiation at energy levels as low as 0.5-1 mJ/mrrr without inducing visible damage to the retina. Expression of other genes evoked by low energy diode laser irradiation is under investigation.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Dec 1 1997|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience