Upregulation of c-fos protein in rat retina induced by sub-visible threshold diode laser treatment

Rong Wen, T. J. Wolfensberge, C. Liu, M. Peng

Research output: Contribution to journalArticle

Abstract

Purpose. This study assesses laser induced biochemical changes, such as the expression of c-fos, an early responsive gene, in the retina after low-energy, subvisible threshold diode laser treatment. Methods. Adult male Long-Evans rats were anesthetized with halothan. Four large (2 mm) spots of laser irradiation were delivered to the retina from a diode laser (810 nm. OcuLight SLx, IRIS Medical Instruments, Inc.) mounted on a dissecting microscope. Retinas were collecfed al various time points after treatmenl. Western blot analysis was performed to determine the protein expression of c-fos. Rgsults. Upregulation of c-fos protein was already detected at an energy level of 0.5 mJ/mnr (2 mm spot size, 50 mW, 30 ms). A! an increased energy level of 1 mJ/mm: (2 mm spot size, 100 mW, 30 ms), induction of c-fos protein was detected as early as 30 min after Ireatment which reached a peak at 4 hr after treatment, and then declined to control level by 8 hr after treatment. No visible change in the retina was observed ophlhalmoscopically during the laser treatment. Conclusions. Our results indicate that expression of c-fos is stimulated in a dynamic fashion by diode laser irradiation at energy levels as low as 0.5-1 mJ/mrrr without inducing visible damage to the retina. Expression of other genes evoked by low energy diode laser irradiation is under investigation.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997
Externally publishedYes

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Proto-Oncogene Proteins c-fos
Semiconductor Lasers
Retina
Up-Regulation
Lasers
Long Evans Rats
Western Blotting
Gene Expression
Genes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Upregulation of c-fos protein in rat retina induced by sub-visible threshold diode laser treatment. / Wen, Rong; Wolfensberge, T. J.; Liu, C.; Peng, M.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 01.12.1997.

Research output: Contribution to journalArticle

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