Up-regulation of murine neonatal T helper cell responses by accessory cell factors

Rebecca D Adkins, Arman Ghanei, Kara Hamilton

Research output: Contribution to journalArticle

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Abstract

We previously found that lymph node T cells from 4-day-old, naive neonatal mice show diminished Th1-like responses. Neonatal T cells produced low levels of IL-2 and proliferated poorly in response to soluble anti-CD3 stimulation. However, neonatal T cells resembled Th2 cells (or primed adult T cells) in that they produced large amounts of IL-4. Here, we have investigated the importance of accessory cell signals in the diminished Th1-like responses of neonatal T cells. Anti-CD28 mAb greatly augmented IL-2 production by neonatal T cells in response to plate-bound anti-CD3 Ab. In response to soluble anti- CD3 mAb plus accessory cells, exogenous accessory cell-derived cytokines were sufficient to restore neonatal responses to adult-like levels. In the presence of exogenous IL-6, IL-4 production by neonatal T cells was largely unchanged whereas IL-2 production was dramatically increased, reaching the high levels produced by adult T cells. In addition, the presence of exogenous IL-6 enhanced the proliferation of neonatal T cells to adult levels. IL-6 also had marked effects on the capacity of neonatal T cells to respond to secondary stimuli. In the absence of exogenous IL-6, neonatal T cells responded poorly to secondary stimulation. This lack of responsiveness was not overcome by the addition of IL-2 or by stimulation with phorbol ester and calcium ionophore. When IL-6 was present during the primary stimulation, neonatal T cells, like adult T cells, produced high levels of IL-4 and proliferated extensively in response to secondary stimuli. Thus, IL-6 was sufficient to restore both the primary and secondary responses of neonatal T cells to mature, adult-like levels. These results imply that neonatal T cells have a greater requirement for accessory cell signals than do adult T cells and may have important bearing in overcoming neonatal T cell immunodeficiencies in vivo.

Original languageEnglish
Pages (from-to)3378-3385
Number of pages8
JournalJournal of Immunology
Volume153
Issue number8
StatePublished - Oct 15 1994

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Helper-Inducer T-Lymphocytes
Up-Regulation
T-Lymphocytes
Interleukin-6
Interleukin-2
Interleukin-4
Th2 Cells
Calcium Ionophores
Phorbol Esters

ASJC Scopus subject areas

  • Immunology

Cite this

Adkins, R. D., Ghanei, A., & Hamilton, K. (1994). Up-regulation of murine neonatal T helper cell responses by accessory cell factors. Journal of Immunology, 153(8), 3378-3385.

Up-regulation of murine neonatal T helper cell responses by accessory cell factors. / Adkins, Rebecca D; Ghanei, Arman; Hamilton, Kara.

In: Journal of Immunology, Vol. 153, No. 8, 15.10.1994, p. 3378-3385.

Research output: Contribution to journalArticle

Adkins, RD, Ghanei, A & Hamilton, K 1994, 'Up-regulation of murine neonatal T helper cell responses by accessory cell factors', Journal of Immunology, vol. 153, no. 8, pp. 3378-3385.
Adkins, Rebecca D ; Ghanei, Arman ; Hamilton, Kara. / Up-regulation of murine neonatal T helper cell responses by accessory cell factors. In: Journal of Immunology. 1994 ; Vol. 153, No. 8. pp. 3378-3385.
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