Up-regulation of micro-RNA-221 (miRNA-221; chr Xp11.3) and caspase-3 accompanies down-regulation of the survivin-1 homolog BIRC1 (NAIP) in glioblastoma multiforme (GBM)

W. J. Lukiw, J. G. Cui, Y. Y. Li, F. Culicchia

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Glioblastoma multiforme (GBM) represents a class of malignant gliomas which rapidly proliferate, invade and destroy surrounding brain tissues. This study examined micro-RNA (miRNA) speciation and miRNA effects on gene expression in six ATCC glioma and GBM cell lines and in 14 glioma and GBM samples obtained from human brain biopsy. We observed selective up-regulation of miRNA-221 and down-regulation of a miRNA-221 messenger RNA target encoding the survivin-1 homolog BIRC1, a neuronal inhibitor of apoptosis protein (NIAP) and marker for neurodegeneration. The expression of BIRC5 (survivin-1) and caspase-3 were found to be significantly up-regulated, particularly in stage IV GBM. These studies suggest that the abundance and speciation of the BIRC family of neural cell fate regulators are differentially regulated in glioma and GBM, and may contribute to progressive changes in apoptotic signaling and altered neural cell cycling functions.

Original languageEnglish (US)
Pages (from-to)27-32
Number of pages6
JournalJournal of neuro-oncology
Volume91
Issue number1
DOIs
StatePublished - Jan 1 2009

Keywords

  • 17q25
  • Apoptosis
  • BIRC (baculoviral IAP repeat-containing)
  • BIRC1
  • BIRC5
  • Caspase-3, chromosome 5q13
  • Glioblastoma multiforme (GBM)
  • Glioma, micro-RNA (miRNA)
  • Inhibitor of apoptosis protein
  • MiRNA-221
  • Neuronal apoptosis inhibitory protein (NAIP)
  • Survivin-1
  • WHO classification
  • Xp11.3

ASJC Scopus subject areas

  • Clinical Neurology
  • Cancer Research
  • Oncology
  • Neurology

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