Up-regulation of interleukin-9 and the interleukin-9-associated calcium-activated chloride channel hCLCA1 in nasal mucosa following in vivo allergen challenge

Hans Peter Hauber, Celine Bergeron, Masao Toda, Mario Kontolemos, Kenneth J. Holroyd, Roy C Levitt, Qutayba Hamid

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Interleukin (IL)-9 is a pleiotropic T helper 2-type cytokine that has been shown to be up-regulated in allergic airway disease, including asthma. IL-9 has been demonstrated to be a potent stimulus for the production and secretion of mucus from airway epithelial cells via induction of a calcium-activated chloride channel, hCLCA1. The objective of this study was to investigate the expression of IL-9 and hCLCA1 following allergen challenge in the nasal mucosa of allergic rhinitis patients. Nasal biopsies were obtained from allergic rhinitis patients out of allergen season both before (baseline) and after local antigen challenge with either ragweed or diluent (control). Immunohistochemistry and in situ hybridization were used to assess IL-9 protein and hCLCA1 messenger ribonucleic acid. Eosinophils and T cells were detected using immunohistochemistry. IL-9 and hCLCA1 were very low at baseline, and expression was significantly up-regulated following ragweed challenge. Whereas the number of eosinophils increased after allergen challenge, T-cell counts did not change significantly. The results of this study demonstrate the relationship between specific allergen challenge and expression of both IL-9 and hCLCA1, suggesting a possible mechanism for the increased production of mucus from airway epithelial cells in allergic rhinitis.

Original languageEnglish
Pages (from-to)19-23
Number of pages5
JournalAllergy, Asthma and Clinical Immunology
Volume3
Issue number1
DOIs
StatePublished - Mar 1 2007
Externally publishedYes

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Interleukin-9
Chloride Channels
Nasal Mucosa
Allergens
Up-Regulation
Ambrosia
Mucus
Eosinophils
Epithelial Cells
Immunohistochemistry
T-Lymphocytes
Nose
In Situ Hybridization
Asthma
Cell Count
RNA
Cytokines
Biopsy
Antigens
Allergic Rhinitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pulmonary and Respiratory Medicine
  • Immunology

Cite this

Up-regulation of interleukin-9 and the interleukin-9-associated calcium-activated chloride channel hCLCA1 in nasal mucosa following in vivo allergen challenge. / Hauber, Hans Peter; Bergeron, Celine; Toda, Masao; Kontolemos, Mario; Holroyd, Kenneth J.; Levitt, Roy C; Hamid, Qutayba.

In: Allergy, Asthma and Clinical Immunology, Vol. 3, No. 1, 01.03.2007, p. 19-23.

Research output: Contribution to journalArticle

Hauber, Hans Peter ; Bergeron, Celine ; Toda, Masao ; Kontolemos, Mario ; Holroyd, Kenneth J. ; Levitt, Roy C ; Hamid, Qutayba. / Up-regulation of interleukin-9 and the interleukin-9-associated calcium-activated chloride channel hCLCA1 in nasal mucosa following in vivo allergen challenge. In: Allergy, Asthma and Clinical Immunology. 2007 ; Vol. 3, No. 1. pp. 19-23.
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