Up-regulation of D3 dopamine receptor mRNA in the nucleus accumbens of human cocaine fatalities

David M. Segal; Carlos T. Moraes; Deborah C. Mash

doi:10.1016/S0169-328X(97)00025-9

Up-regulation of D₃ dopamine receptor mRNA in the nucleus accumbens of human cocaine fatalities

David M. Segal, Carlos T. Moraes, Deborah C. Mash

Neurology

Research output: Contribution to journal › Article › peer-review

133 Scopus citations

Abstract

The effects of chronic cocaine use on D₃ receptor mRNA expression in the human nucleus accumbens was assessed by reverse transcription-polymerase chain reaction. D₃ receptor/cyclophilin mRNA ratios in the nucleus accumbens were increased 6-fold in cocaine overdose victims as compared to age-matched and drug-free control subjects. This finding demonstrates that chronic cocaine exposure leads to adaptive increases in the expression of D₃ receptor mRNA in a critical reward center in brain.

Original language	English (US)
Pages (from-to)	335-339
Number of pages	5
Journal	Molecular Brain Research
Volume	45
Issue number	2
DOIs	https://doi.org/10.1016/S0169-328X(97)00025-9
State	Published - May 1997

Keywords

Cocaine
Dopamine
Excited delirium
Human brain
Polymerase chain reaction
Receptor
Reverse transcription
Ribonuclease protection assay

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

Access to Document

10.1016/S0169-328X(97)00025-9

Cite this

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title = "Up-regulation of D3 dopamine receptor mRNA in the nucleus accumbens of human cocaine fatalities",

abstract = "The effects of chronic cocaine use on D3 receptor mRNA expression in the human nucleus accumbens was assessed by reverse transcription-polymerase chain reaction. D3 receptor/cyclophilin mRNA ratios in the nucleus accumbens were increased 6-fold in cocaine overdose victims as compared to age-matched and drug-free control subjects. This finding demonstrates that chronic cocaine exposure leads to adaptive increases in the expression of D3 receptor mRNA in a critical reward center in brain.",

keywords = "Cocaine, Dopamine, Excited delirium, Human brain, Polymerase chain reaction, Receptor, Reverse transcription, Ribonuclease protection assay",

author = "Segal, {David M.} and Moraes, {Carlos T.} and Mash, {Deborah C.}",

note = "Funding Information: We wish to thank Margaret Basile for her expert technical assistance. We thank W. Lee Hearn (Dade County Medical Examiner Department, Dade County, FL) for conducting toxicology analysis of brain tissues. This study was supported by NIDA Grant DA06227.",

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AU - Segal, David M.

AU - Moraes, Carlos T.

AU - Mash, Deborah C.

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KW - Cocaine

KW - Dopamine

KW - Excited delirium

KW - Human brain

KW - Polymerase chain reaction

KW - Receptor

KW - Reverse transcription

KW - Ribonuclease protection assay

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