Unusual retention of rhodamine 123 by mitochondria in muscle and carcinoma cells

I. C. Summerhayes, Theodore Lampidis, S. D. Bernal, J. J. Nadakavukaren, K. K. Nadakavukaren, E. L. Shepherd, L. B. Chen

Research output: Contribution to journalArticle

321 Citations (Scopus)

Abstract

Mitochondria in cardiac muscle cells and myoblast-fused myotubes display unusually long (3-5 days) retention times of rhodamine 123, a mitochondria-specific fluorescent probe, in living cells. Among 50 keratin-positive carcinoma or transformed epithelial cell lines tested, mitochondria with prolonged rhodamine 123 retention are detected in most of the transitional cell carcinoma, adenocarcinoma, and chemical carcinogen-transformed epithelial cell lines and in some squamous cell carcinoma lines but not in any oat cell carcinoma lines. The presence of mitochondria having unusual dye retention may be useful for diagnosis and exploitable for chemotherapy of certain human carcinomas.

Original languageEnglish
Pages (from-to)5292-5296
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number17 I
DOIs
StatePublished - Dec 1 1982
Externally publishedYes

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Rhodamine 123
Muscle Cells
Mitochondria
Carcinoma
Transformed Cell Line
Cardiac Myoblasts
Epithelial Cells
Cell Line
Small Cell Carcinoma
Transitional Cell Carcinoma
Skeletal Muscle Fibers
Keratins
Fluorescent Dyes
Cardiac Myocytes
Carcinogens
Squamous Cell Carcinoma
Adenocarcinoma
Coloring Agents
Drug Therapy

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Summerhayes, I. C., Lampidis, T., Bernal, S. D., Nadakavukaren, J. J., Nadakavukaren, K. K., Shepherd, E. L., & Chen, L. B. (1982). Unusual retention of rhodamine 123 by mitochondria in muscle and carcinoma cells. Proceedings of the National Academy of Sciences of the United States of America, 79(17 I), 5292-5296. https://doi.org/10.1073/pnas.79.17.5292

Unusual retention of rhodamine 123 by mitochondria in muscle and carcinoma cells. / Summerhayes, I. C.; Lampidis, Theodore; Bernal, S. D.; Nadakavukaren, J. J.; Nadakavukaren, K. K.; Shepherd, E. L.; Chen, L. B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, No. 17 I, 01.12.1982, p. 5292-5296.

Research output: Contribution to journalArticle

Summerhayes, I. C. ; Lampidis, Theodore ; Bernal, S. D. ; Nadakavukaren, J. J. ; Nadakavukaren, K. K. ; Shepherd, E. L. ; Chen, L. B. / Unusual retention of rhodamine 123 by mitochondria in muscle and carcinoma cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1982 ; Vol. 79, No. 17 I. pp. 5292-5296.
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