Unmethylated E-cadherin gene expressionis significantly associated with metastatic human prostate cancer cells in bone

Baisakhi Saha, Pavinder Kaur, Denice Tsao-Wei, Wesley Y. Naritoku, Susan Groshen, Ram H. Datar, Lawrence W. Jones, S. Ashraf Imam

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

BACKGROUND. The concurrent determination of methylation status of E-cadherin gene and E-cadherin protein expression remains scant in metastatic prostate cancer cells in bone, the most prevalent site for metastatic growth. Therefore, the study was undertaken to ascertain the methylation status of E-cadherin gene, a most frequent and known epigenetic mechanism of its regulation, and the protein expression in prostate tissue biopsy specimen. METHODS. The methylation of E-cadherin gene was determined by methylation specific-PCR and the protein expression by immunohistochemical method in the consecutive sections of each prostate tissue biopsy specimen. RESULTS. The unmethylated E-cadherin gene and homogeneous E-cadherin protein expression was significantly associated with BPH as compared to the primary prostate carcinoma (Fisher's Exact P<0.001). A significant association was observed between the concurrent methylated gene and markedly reduced expression of the protein in the primary prostate cancer cells as compared to the BPH cells, suggesting methylation-dependent regulation of the gene expression in these cases. In contrast to the primary cancer, a highly significant increase in the frequency of metastatic prostate cancer cells in bone exhibited the concurrent expression of unmethylated gene and homogeneous protein (Fisher's Exact P<0.001). CONCLUSIONS. The study clearly demonstrated a significant association of the concurrent expression of unmethylated E-cadherin gene and E-cadherin protein with metastatic prostate cancer cells in bone, and that its expression may have a role in the intercellular adhesion in the formation of metastatic lesions in bone.

Original languageEnglish (US)
Pages (from-to)1681-1688
Number of pages8
JournalProstate
Volume68
Issue number15
DOIs
StatePublished - Nov 1 2008

Keywords

  • Bone metastasis
  • Immunohistochemistry
  • Methylated E-cadherin gene
  • MS-PCR
  • Prostate cancer

ASJC Scopus subject areas

  • Urology
  • Oncology
  • Medicine(all)

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    Saha, B., Kaur, P., Tsao-Wei, D., Naritoku, W. Y., Groshen, S., Datar, R. H., Jones, L. W., & Imam, S. A. (2008). Unmethylated E-cadherin gene expressionis significantly associated with metastatic human prostate cancer cells in bone. Prostate, 68(15), 1681-1688. https://doi.org/10.1002/pros.20836