Unitary assembly of presynaptic active zones from Piccolo-Bassoon transport vesicles

Mika Shapira, Rong G Zhai, Thomas Dresbach, Tal Bresler, Viviana I. Torres, Eckart D. Gundelfinger, Noam E. Ziv, Craig C. Garner

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

Recent studies indicate that active zones (AZs) - sites of neurotransmitter release - may be assembled from preassembled AZ precursor vesicles inserted into the presynaptic plasma membrane. Here we report that one putative AZ precursor vesicle of CNS synapses - the Piccolo-Bassoon transport vesicle (PTV) - carries a comprehensive set of AZ proteins genetically and functionally coupled to synaptic vesicle exocytosis. Time-lapse imaging reveals that PTVs are highly mobile, consistent with a role in intracellular transport. Quantitative analysis reveals that the Bassoon, Piccolo, and RIM content of individual PTVs is, on average, half of that of individual presynaptic boutons and shows that the synaptic content of these molecules can be quantitatively accounted for by incorporation of integer numbers (typically two to three) of PTVs into presynaptic membranes. These findings suggest that AZs are assembled from unitary amounts of AZ material carried on PTVs.

Original languageEnglish
Pages (from-to)237-252
Number of pages16
JournalNeuron
Volume38
Issue number2
DOIs
StatePublished - Apr 24 2003
Externally publishedYes

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Time-Lapse Imaging
Transport Vesicles
Synaptic Vesicles
Exocytosis
Presynaptic Terminals
Synapses
Neurotransmitter Agents
Catalytic Domain
Cell Membrane
Membranes
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Shapira, M., Zhai, R. G., Dresbach, T., Bresler, T., Torres, V. I., Gundelfinger, E. D., ... Garner, C. C. (2003). Unitary assembly of presynaptic active zones from Piccolo-Bassoon transport vesicles. Neuron, 38(2), 237-252. https://doi.org/10.1016/S0896-6273(03)00207-1

Unitary assembly of presynaptic active zones from Piccolo-Bassoon transport vesicles. / Shapira, Mika; Zhai, Rong G; Dresbach, Thomas; Bresler, Tal; Torres, Viviana I.; Gundelfinger, Eckart D.; Ziv, Noam E.; Garner, Craig C.

In: Neuron, Vol. 38, No. 2, 24.04.2003, p. 237-252.

Research output: Contribution to journalArticle

Shapira, M, Zhai, RG, Dresbach, T, Bresler, T, Torres, VI, Gundelfinger, ED, Ziv, NE & Garner, CC 2003, 'Unitary assembly of presynaptic active zones from Piccolo-Bassoon transport vesicles', Neuron, vol. 38, no. 2, pp. 237-252. https://doi.org/10.1016/S0896-6273(03)00207-1
Shapira M, Zhai RG, Dresbach T, Bresler T, Torres VI, Gundelfinger ED et al. Unitary assembly of presynaptic active zones from Piccolo-Bassoon transport vesicles. Neuron. 2003 Apr 24;38(2):237-252. https://doi.org/10.1016/S0896-6273(03)00207-1
Shapira, Mika ; Zhai, Rong G ; Dresbach, Thomas ; Bresler, Tal ; Torres, Viviana I. ; Gundelfinger, Eckart D. ; Ziv, Noam E. ; Garner, Craig C. / Unitary assembly of presynaptic active zones from Piccolo-Bassoon transport vesicles. In: Neuron. 2003 ; Vol. 38, No. 2. pp. 237-252.
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