Unexpected diversity of cellular immune responses against nef and Vif in HIV-1-infected patients who spontaneously control viral replication

Leandro F. Tarosso, Mariana M. Sauer, Sabri Sanabani, Maria Teresa Giret, Helena I. Tomiyama, John Sidney, Shari M. Piaskowski, Ricardo S. Diaz, Ester C. Sabino, Alessandro Sette, Jorge Kalil-Filho, David Watkins, Esper G. Kallas

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: HIV-1-infected individuals who spontaneously control viral replication represent an example of successful containment of the AIDS virus. Understanding the anti-viral immune responses in these individuals may help in vaccine design. However, immune responses against HIV-1 are normally analyzed using HIV-1 consensus B 15-mers that overlap by 11 amino acids. Unfortunately, this method may underestimate the real breadth of the cellular immune responses against the autologous sequence of the infecting virus.Methodology and Principal Findings: Here we compared cellular immune responses against nef and vif-encoded consensus B 15-mer peptides to responses against HLA class I-predicted minimal optimal epitopes from consensus B and autologous sequences in six patients who have controlled HIV-1 replication. Interestingly, our analysis revealed that three of our patients had broader cellular immune responses against HLA class I-predicted minimal optimal epitopes from either autologous viruses or from the HIV-1 consensus B sequence, when compared to responses against the 15-mer HIV-1 type B consensus peptides. Conclusion and Significance: This suggests that the cellular immune responses against HIV-1 in controller patients may be broader than we had previously anticipated.

Original languageEnglish
Article numbere11436
JournalPLoS One
Volume5
Issue number7
DOIs
StatePublished - Aug 13 2010
Externally publishedYes

Fingerprint

virus replication
Human immunodeficiency virus 1
Viruses
Cellular Immunity
cell-mediated immunity
HIV-1
Epitopes
Peptides
viruses
Vaccines
epitopes
Amino Acids
immune response
Controllers
peptides
Consensus Sequence
controllers
HIV
vaccines
amino acids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tarosso, L. F., Sauer, M. M., Sanabani, S., Giret, M. T., Tomiyama, H. I., Sidney, J., ... Kallas, E. G. (2010). Unexpected diversity of cellular immune responses against nef and Vif in HIV-1-infected patients who spontaneously control viral replication. PLoS One, 5(7), [e11436]. https://doi.org/10.1371/journal.pone.0011436

Unexpected diversity of cellular immune responses against nef and Vif in HIV-1-infected patients who spontaneously control viral replication. / Tarosso, Leandro F.; Sauer, Mariana M.; Sanabani, Sabri; Giret, Maria Teresa; Tomiyama, Helena I.; Sidney, John; Piaskowski, Shari M.; Diaz, Ricardo S.; Sabino, Ester C.; Sette, Alessandro; Kalil-Filho, Jorge; Watkins, David; Kallas, Esper G.

In: PLoS One, Vol. 5, No. 7, e11436, 13.08.2010.

Research output: Contribution to journalArticle

Tarosso, LF, Sauer, MM, Sanabani, S, Giret, MT, Tomiyama, HI, Sidney, J, Piaskowski, SM, Diaz, RS, Sabino, EC, Sette, A, Kalil-Filho, J, Watkins, D & Kallas, EG 2010, 'Unexpected diversity of cellular immune responses against nef and Vif in HIV-1-infected patients who spontaneously control viral replication', PLoS One, vol. 5, no. 7, e11436. https://doi.org/10.1371/journal.pone.0011436
Tarosso, Leandro F. ; Sauer, Mariana M. ; Sanabani, Sabri ; Giret, Maria Teresa ; Tomiyama, Helena I. ; Sidney, John ; Piaskowski, Shari M. ; Diaz, Ricardo S. ; Sabino, Ester C. ; Sette, Alessandro ; Kalil-Filho, Jorge ; Watkins, David ; Kallas, Esper G. / Unexpected diversity of cellular immune responses against nef and Vif in HIV-1-infected patients who spontaneously control viral replication. In: PLoS One. 2010 ; Vol. 5, No. 7.
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