Uncoupling of local cerebral glucose metabolism and blood flow after acute fluid-percussion injury in rats

M. D. Ginsberg, W. Zhao, O. F. Alonso, J. Y. Loor-Estades, W. D. Dietrich, R. Busto

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

We assessed local cerebral glucose metabolism (1CMR(Glc)) and blood flow (1CBF) interrelationships in the first hour after parasagittal fluid- percussion head injury (FPI) in rats. Matched series were studied autoradiographically for 1CMR(Glc) and 1CBF with 2-[14C]deoxyglucose and 14C-labeled iodoantipyrine, respectively. Three-dimensional autoradiographic-image mapping was used to generate average data sets from which a mean 1CMR(Glc)-to-1CBF ratio data set was derived. 1CBF in neocortical regions ipsilateral to the trauma were depressed, on average, by 44% compared with sham-FPI rats, whereas contralateral 1CBF values were not altered. By contrast, 1CMR(Glc) was elevated in many cortical and subcortical sites of both hemispheres; this amounted to 1.3- to 1.4-fold increases in neocortical regions in the thalamus and 1.6- to 1.7-fold increases in the hippocampus. The 1CMR(Glc)-to-1CBF ratio data revealed striking elevations both ipsilateral (P = 7 x 10-7) and contralateral to the FPI (P = 0.003). The extent of metabolism-flow uncoupling, on average, amounted to 2.5-fold in the ipsilateral hippocampus and neocortex and 1.7-fold contralaterally. The loci of pronounced metabolism-flow dissociation corresponded closely to the previously documented histological distribution of neuronal necrosis. Our findings resemble events occurring in the acute focal ischemic penumbra and suggest that similar injury mechanisms may be operative.

Original languageEnglish (US)
Pages (from-to)H2859-H2868
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume272
Issue number6 41-6
DOIs
StatePublished - 1997

Keywords

  • Autoradiography
  • Coupling
  • Glycolysis
  • Head injury
  • Image processing

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Uncoupling of local cerebral glucose metabolism and blood flow after acute fluid-percussion injury in rats'. Together they form a unique fingerprint.

  • Cite this