TY - JOUR
T1 - Unconventional secretion of tubby and tubby-like protein 1
AU - Caberoy, Nora B.
AU - Li, Wei
N1 - Funding Information:
We are grateful to Dr. Douglas Vollrath for discussion. This project was supported in part by NIH R01EY016211., P30-EY014801 and Research to Prevent Blindness. N.B.C. is a recipient of a Postdoctoral Fellowship from Fight-for-Sight.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2009/9/17
Y1 - 2009/9/17
N2 - Tubby-like proteins (Tulps) with no signal peptide have been characterized as cytoplasmic proteins with various intracellular functions, including binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PI(4,5)P2 has been implicated in unconventional secretion of fibroblast growth factor-2 without a signal peptide. Here, we show that all Tulps are expressed intracellularly and extracellularly. Tubby secretion is partially dependent on its PI(4,5)P2-binding activity with an essential secretory signal in the N-terminus. Pathogenic mutation in Tubby mice has no impact on tubby extracellular trafficking. Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well.
AB - Tubby-like proteins (Tulps) with no signal peptide have been characterized as cytoplasmic proteins with various intracellular functions, including binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PI(4,5)P2 has been implicated in unconventional secretion of fibroblast growth factor-2 without a signal peptide. Here, we show that all Tulps are expressed intracellularly and extracellularly. Tubby secretion is partially dependent on its PI(4,5)P2-binding activity with an essential secretory signal in the N-terminus. Pathogenic mutation in Tubby mice has no impact on tubby extracellular trafficking. Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well.
KW - Extracellular trafficking
KW - Obesity
KW - Retinal degeneration
KW - Tubby
KW - Tubby-like protein 1
KW - Unconventional secretion
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U2 - 10.1016/j.febslet.2009.08.015
DO - 10.1016/j.febslet.2009.08.015
M3 - Article
C2 - 19695251
AN - SCOPUS:69949122067
VL - 583
SP - 3057
EP - 3062
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 18
ER -
