Unconventional secretion of tubby and tubby-like protein 1

Nora B. Caberoy, Wei Li

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tubby-like proteins (Tulps) with no signal peptide have been characterized as cytoplasmic proteins with various intracellular functions, including binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PI(4,5)P2 has been implicated in unconventional secretion of fibroblast growth factor-2 without a signal peptide. Here, we show that all Tulps are expressed intracellularly and extracellularly. Tubby secretion is partially dependent on its PI(4,5)P2-binding activity with an essential secretory signal in the N-terminus. Pathogenic mutation in Tubby mice has no impact on tubby extracellular trafficking. Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well.

Original languageEnglish
Pages (from-to)3057-3062
Number of pages6
JournalFEBS Letters
Volume583
Issue number18
DOIs
StatePublished - Sep 17 2009

Fingerprint

Protein Sorting Signals
Proteins
Fibroblast Growth Factor 2
Phosphatidylinositols
Endoplasmic Reticulum
Mutation

Keywords

  • Extracellular trafficking
  • Obesity
  • Retinal degeneration
  • Tubby
  • Tubby-like protein 1
  • Unconventional secretion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Unconventional secretion of tubby and tubby-like protein 1. / Caberoy, Nora B.; Li, Wei.

In: FEBS Letters, Vol. 583, No. 18, 17.09.2009, p. 3057-3062.

Research output: Contribution to journalArticle

Caberoy, Nora B. ; Li, Wei. / Unconventional secretion of tubby and tubby-like protein 1. In: FEBS Letters. 2009 ; Vol. 583, No. 18. pp. 3057-3062.
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