Abstract
Tubby-like proteins (Tulps) with no signal peptide have been characterized as cytoplasmic proteins with various intracellular functions, including binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PI(4,5)P2 has been implicated in unconventional secretion of fibroblast growth factor-2 without a signal peptide. Here, we show that all Tulps are expressed intracellularly and extracellularly. Tubby secretion is partially dependent on its PI(4,5)P2-binding activity with an essential secretory signal in the N-terminus. Pathogenic mutation in Tubby mice has no impact on tubby extracellular trafficking. Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well.
| Original language | English |
|---|---|
| Pages (from-to) | 3057-3062 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 583 |
| Issue number | 18 |
| DOIs | |
| State | Published - Sep 17 2009 |
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Keywords
- Extracellular trafficking
- Obesity
- Retinal degeneration
- Tubby
- Tubby-like protein 1
- Unconventional secretion
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Genetics
- Molecular Biology
- Structural Biology
Cite this
Unconventional secretion of tubby and tubby-like protein 1. / Caberoy, Nora B.; Li, Wei.
In: FEBS Letters, Vol. 583, No. 18, 17.09.2009, p. 3057-3062.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Unconventional secretion of tubby and tubby-like protein 1
AU - Caberoy, Nora B.
AU - Li, Wei
PY - 2009/9/17
Y1 - 2009/9/17
N2 - Tubby-like proteins (Tulps) with no signal peptide have been characterized as cytoplasmic proteins with various intracellular functions, including binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PI(4,5)P2 has been implicated in unconventional secretion of fibroblast growth factor-2 without a signal peptide. Here, we show that all Tulps are expressed intracellularly and extracellularly. Tubby secretion is partially dependent on its PI(4,5)P2-binding activity with an essential secretory signal in the N-terminus. Pathogenic mutation in Tubby mice has no impact on tubby extracellular trafficking. Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well.
AB - Tubby-like proteins (Tulps) with no signal peptide have been characterized as cytoplasmic proteins with various intracellular functions, including binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]. PI(4,5)P2 has been implicated in unconventional secretion of fibroblast growth factor-2 without a signal peptide. Here, we show that all Tulps are expressed intracellularly and extracellularly. Tubby secretion is partially dependent on its PI(4,5)P2-binding activity with an essential secretory signal in the N-terminus. Pathogenic mutation in Tubby mice has no impact on tubby extracellular trafficking. Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well.
KW - Extracellular trafficking
KW - Obesity
KW - Retinal degeneration
KW - Tubby
KW - Tubby-like protein 1
KW - Unconventional secretion
UR - http://www.scopus.com/inward/record.url?scp=69949122067&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=69949122067&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2009.08.015
DO - 10.1016/j.febslet.2009.08.015
M3 - Article
C2 - 19695251
AN - SCOPUS:69949122067
VL - 583
SP - 3057
EP - 3062
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 18
ER -