Abstract
Prematurity is a risk factor for hypertension, vascular stiffness, nephron deficit and adult onset cardiorenal disease. The vascular tree and kidneys share morphogenic drivers that promote maturation in utero before 36 weeks of gestation. Vascular elastin accrual terminates after birth leaving collagen to promote vascular stiffness. Our objective was to determine if the histomorphometry of the umbilical artery, an extension of the aorta, parallels nephron mass across gestational age groups. From a cohort of 54 newborns, 32 umbilical cord specimens were adequate for evaluation. The umbilical cord was sectioned, stained with trichrome, and digitalized. Muscular and collagenous areas of the umbilical artery were measured in pixels using the Image J 1.48q software. Total kidney volume was measured by ultrasound and factored by body surface area (TKV/BSA). The umbilical artery total area was significantly greater in term v. preterm infants (9.3±1.3 v. 7.0±2.0 mm2; P<0.05) and increased with gestational age; while the percent muscular and collagen areas were independent of gestational age (R 2=0.04; P=ns). Percent muscular area correlated positively with TKV/BSA (r=0.53; P=0.002); while an increase in collagen correlated inversely with kidney mass (r=−0.53; P=0.002). In conclusion, an enhanced % muscular area and presumed vascular elasticity was associated with increased renal mass in all infants. Umbilical artery histomorphometry provides a link between the intrauterine environment, vascular and kidney development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | Journal of Developmental Origins of Health and Disease |
| DOIs | |
| State | Accepted/In press - Mar 6 2017 |
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Keywords
- cardiorenal disease
- kidney development
- prematurity
- vascular development
ASJC Scopus subject areas
- Medicine (miscellaneous)
Cite this
Umbilical artery histomorphometry : a link between the intrauterine environment and kidney development. / DeFreitas, M. J.; Mathur, D.; Seeherunvong, Wacharee; Cano, T.; Katsoufis, Chryso; Duara, Shahnaz; Yasin, Salih Y; Zilleruelo, Gaston E; Rodriguez, Maria; Abitbol, Carolyn.
In: Journal of Developmental Origins of Health and Disease, 06.03.2017, p. 1-8.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Umbilical artery histomorphometry
T2 - a link between the intrauterine environment and kidney development
AU - DeFreitas, M. J.
AU - Mathur, D.
AU - Seeherunvong, Wacharee
AU - Cano, T.
AU - Katsoufis, Chryso
AU - Duara, Shahnaz
AU - Yasin, Salih Y
AU - Zilleruelo, Gaston E
AU - Rodriguez, Maria
AU - Abitbol, Carolyn
PY - 2017/3/6
Y1 - 2017/3/6
N2 - Prematurity is a risk factor for hypertension, vascular stiffness, nephron deficit and adult onset cardiorenal disease. The vascular tree and kidneys share morphogenic drivers that promote maturation in utero before 36 weeks of gestation. Vascular elastin accrual terminates after birth leaving collagen to promote vascular stiffness. Our objective was to determine if the histomorphometry of the umbilical artery, an extension of the aorta, parallels nephron mass across gestational age groups. From a cohort of 54 newborns, 32 umbilical cord specimens were adequate for evaluation. The umbilical cord was sectioned, stained with trichrome, and digitalized. Muscular and collagenous areas of the umbilical artery were measured in pixels using the Image J 1.48q software. Total kidney volume was measured by ultrasound and factored by body surface area (TKV/BSA). The umbilical artery total area was significantly greater in term v. preterm infants (9.3±1.3 v. 7.0±2.0 mm2; P<0.05) and increased with gestational age; while the percent muscular and collagen areas were independent of gestational age (R 2=0.04; P=ns). Percent muscular area correlated positively with TKV/BSA (r=0.53; P=0.002); while an increase in collagen correlated inversely with kidney mass (r=−0.53; P=0.002). In conclusion, an enhanced % muscular area and presumed vascular elasticity was associated with increased renal mass in all infants. Umbilical artery histomorphometry provides a link between the intrauterine environment, vascular and kidney development.
AB - Prematurity is a risk factor for hypertension, vascular stiffness, nephron deficit and adult onset cardiorenal disease. The vascular tree and kidneys share morphogenic drivers that promote maturation in utero before 36 weeks of gestation. Vascular elastin accrual terminates after birth leaving collagen to promote vascular stiffness. Our objective was to determine if the histomorphometry of the umbilical artery, an extension of the aorta, parallels nephron mass across gestational age groups. From a cohort of 54 newborns, 32 umbilical cord specimens were adequate for evaluation. The umbilical cord was sectioned, stained with trichrome, and digitalized. Muscular and collagenous areas of the umbilical artery were measured in pixels using the Image J 1.48q software. Total kidney volume was measured by ultrasound and factored by body surface area (TKV/BSA). The umbilical artery total area was significantly greater in term v. preterm infants (9.3±1.3 v. 7.0±2.0 mm2; P<0.05) and increased with gestational age; while the percent muscular and collagen areas were independent of gestational age (R 2=0.04; P=ns). Percent muscular area correlated positively with TKV/BSA (r=0.53; P=0.002); while an increase in collagen correlated inversely with kidney mass (r=−0.53; P=0.002). In conclusion, an enhanced % muscular area and presumed vascular elasticity was associated with increased renal mass in all infants. Umbilical artery histomorphometry provides a link between the intrauterine environment, vascular and kidney development.
KW - cardiorenal disease
KW - kidney development
KW - prematurity
KW - vascular development
UR - http://www.scopus.com/inward/record.url?scp=85014566483&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014566483&partnerID=8YFLogxK
U2 - 10.1017/S2040174417000113
DO - 10.1017/S2040174417000113
M3 - Article
C2 - 28260559
AN - SCOPUS:85014566483
SP - 1
EP - 8
JO - Journal of Developmental Origins of Health and Disease
JF - Journal of Developmental Origins of Health and Disease
SN - 2040-1744
ER -