Purpose. To establish the transmittance of the lens capsule to ultraviolet radiation. Methods. New Zeeland albino rabbits were enucleated on both sides. The lens was extracted and the posterior capsule was mounted for spectroscopy in a double beam spectrophotometer The transmittance from 250 up to 450 nm was measured differentially for axially transmitted light and for all light transmitted. All light transmitted was recorded with an integrating sphere. During the measurement, the capsule was kept in a quartz cuvette containing BSS. Results. The total transmittance was on the order of 0.7 at 250 nm, decreased slightly up to 280 nm and then increased to allmost 1 at around 300 nm. The direct transmittance was on the order of 0.3 at 250 nm, remained fairly constant up to 280 nm, increased to approximately 0.6 at 300 nm and then increased allmost linearly to approximately 0.75 at 450 nm. Conclusions. The lens capsule is highly transmitting at around 300 nm. The transmittance spectrum below 300 nm corresponds to protein absorption and scattering. The measurements indicates an increased scattering towards lower wavelenghts.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Feb 15 1996|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience