Ultrastructural observations of granule extrusion from rat anterior pituitary cells after injection of LH-releasing hormone

M. Shiino, A. Arimura, A. V. Schally, E. G. Rennels

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In order to observe the ultrastructural aspects of granule extrusion from gonadotrophs, the authors injected pure, natural porcine LH-releasing hormone (LH-RH) into persistent-estrous (PE) rats. Clear-cut extrusions of secretory granules appeared from LH-gonadotrophs 2.5, 5 and 15 minutes after the injection. Massive extrusion was observed at 15 minutes. Serum LH and FSH were also estimated by radioimmunoassay on blood samples taken at the same times after the injections. LH was increased in the blood sera after the injection of LH-RH, but serum FSH was not significantly different among the various treated rats. The rise in serum LH after LH-RH injection was well correlated with the ultrastructural phenomenon of granule extrusion from LH-gonadotrophs.

Original languageEnglish (US)
Pages (from-to)152-161
Number of pages10
JournalZeitschrift für Zellforschung und Mikroskopische Anatomie
Issue number2
StatePublished - Jun 1 1972
Externally publishedYes



  • Granule extrusion
  • LH-releasing hormone
  • Rat pituitary gland
  • Ultrastructure

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology
  • Medicine(all)

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