Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain

Zsolt Liposits; Tamás Görcs; György Sétáló; István Lengvári; Béla Flerkó; Sándor Vigh; Andrew V. Schally

doi:10.1007/BF00217891

Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain

Zsolt Liposits, Tamás Görcs, György Sétáló, István Lengvári, Béla Flerkó, Sándor Vigh, Andrew V. Schally

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.

Original language	English (US)
Pages (from-to)	191-196
Number of pages	6
Journal	Cell and Tissue Research
Volume	229
Issue number	1
DOIs	https://doi.org/10.1007/BF00217891
State	Published - Jan 1 1983
Externally published	Yes

Keywords

CRF
Electron microscopy
immunolabelling
Median eminence
Paraventricular nucleus

ASJC Scopus subject areas

Anatomy
Clinical Biochemistry
Cell Biology

Access to Document

10.1007/BF00217891

Fingerprint Dive into the research topics of 'Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain'. Together they form a unique fingerprint.

Cite this

Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain. / Liposits, Zsolt; Görcs, Tamás; Sétáló, György; Lengvári, István; Flerkó, Béla; Vigh, Sándor; Schally, Andrew V.

In: Cell and Tissue Research, Vol. 229, No. 1, 01.01.1983, p. 191-196.

Research output: Contribution to journal › Article › peer-review

@article{1afef3c1d244414bab535042d5aa373e,

title = "Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain",

abstract = "The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.",

keywords = "CRF, Electron microscopy, immunolabelling, Median eminence, Paraventricular nucleus",

author = "Zsolt Liposits and Tam{\'a}s G{\"o}rcs and Gy{\"o}rgy S{\'e}t{\'a}l{\'o} and Istv{\'a}n Lengv{\'a}ri and B{\'e}la Flerk{\'o} and S{\'a}ndor Vigh and Schally, {Andrew V.}",

year = "1983",

month = jan,

day = "1",

doi = "10.1007/BF00217891",

language = "English (US)",

volume = "229",

pages = "191--196",

journal = "Cell and Tissue Research",

issn = "0302-766X",

publisher = "Springer Verlag",

number = "1",

}

TY - JOUR

T1 - Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain

AU - Liposits, Zsolt

AU - Görcs, Tamás

AU - Sétáló, György

AU - Lengvári, István

AU - Flerkó, Béla

AU - Vigh, Sándor

AU - Schally, Andrew V.

PY - 1983/1/1

Y1 - 1983/1/1

N2 - The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.

AB - The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.

KW - CRF

KW - Electron microscopy

KW - immunolabelling

KW - Median eminence

KW - Paraventricular nucleus

UR - http://www.scopus.com/inward/record.url?scp=0020674927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020674927&partnerID=8YFLogxK

U2 - 10.1007/BF00217891

DO - 10.1007/BF00217891

M3 - Article

C2 - 6600971

AN - SCOPUS:0020674927

VL - 229

SP - 191

EP - 196

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 1

ER -