Abstract
The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.
Original language | English (US) |
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Pages (from-to) | 191-196 |
Number of pages | 6 |
Journal | Cell and Tissue Research |
Volume | 229 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1983 |
Externally published | Yes |
Keywords
- CRF
- Electron microscopy
- immunolabelling
- Median eminence
- Paraventricular nucleus
ASJC Scopus subject areas
- Anatomy
- Clinical Biochemistry
- Cell Biology