Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain

Zsolt Liposits; Tamás Görcs; György Sétáló; István Lengvári; Béla Flerkó; Sándor Vigh; Andrew V. Schally

doi:10.1007/BF00217891

Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain

Zsolt Liposits, Tamás Görcs, György Sétáló, István Lengvári, Béla Flerkó, Sándor Vigh, Andrew V. Schally

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.

Original language	English (US)
Pages (from-to)	191-196
Number of pages	6
Journal	Cell and Tissue Research
Volume	229
Issue number	1
DOIs	https://doi.org/10.1007/BF00217891
State	Published - Jan 1983
Externally published	Yes

Keywords

CRF
Electron microscopy
immunolabelling
Median eminence
Paraventricular nucleus

ASJC Scopus subject areas

Anatomy
Clinical Biochemistry
Cell Biology

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10.1007/BF00217891

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Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain. / Liposits, Zsolt; Görcs, Tamás; Sétáló, György; Lengvári, István; Flerkó, Béla; Vigh, Sándor; Schally, Andrew V.

In: Cell and Tissue Research, Vol. 229, No. 1, 01.1983, p. 191-196.

Research output: Contribution to journal › Article › peer-review

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abstract = "The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.",

keywords = "CRF, Electron microscopy, immunolabelling, Median eminence, Paraventricular nucleus",

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AU - Sétáló, György

AU - Lengvári, István

AU - Flerkó, Béla

AU - Vigh, Sándor

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AB - The corticotropin releasing factor (CRF)-synthesizing perikarya and neural processes were detected at ultrastructural level in the hypothalamic paraventricular nucleus and in the median eminence of control and colchicine-pretreated rats. The unlabelled antibody peroxidase-antiperoxidase complex (PAP) immunohistochemical method was used in a pre-embedding manner, on thick, non-frozen sections. In CRF-perikarya, neurosecretory granules (80-120 nm in diameter), free ribosomes, and the rough endoplasmic reticulum were labelled. Unlabelled axon terminals formed asymmetric synapses on CRF-containing perikarya and dendrites. Immunolabelled axons terminated in the palisadic zone of the median eminence.

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KW - Electron microscopy

KW - immunolabelling

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KW - Paraventricular nucleus

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Ultrastructural characteristics of immunolabelled, corticotropin releasing factor (CRF)-synthesizing neurons in the rat brain

Abstract

Keywords

ASJC Scopus subject areas

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