TY - JOUR
T1 - Ultrastructural and light microscopic observations of rat pituitary LH-containing gonadotrophs following injection of synthetic LH-RH
AU - Mendoza, D.
AU - Arimura, A.
AU - Schally, A. V.
PY - 1973/4
Y1 - 1973/4
N2 - The effect of luteinizing hormonereleasing hormone (LH-RH) on pituitary cytology was investigated in intact, hemicastrated, and castrated male and female rats using electron and light microscopy. Two hundred ng of LH-RH were injected into the carotid artery, and the animals were sacrificed 1, 3, and 9 min after the injection. Study of LH-containing gonadotrophs by an immunocytochemical method using peroxidase- labeled antibody showed depletion of peroxidase reactivity of LH cells 1 min after injection of LH-RH in intact and hemicastrated proestrous rats, and after 9 min in the castrated females. Serum LH levels measured by radioimmunoassay were found to be significantly elevated 3 and 9 min after LH-RH injection in intact, hemicastrated and castrated male and female rats. Serum FSH levels had risen 1, 3, and 9 min after injection of LH-RH in the intact males and hemicastrated proestrous females, had fallen 1 and 3 min after injection in the hemicastrated male and had not changed 1, 3, or 9 min after injection in the castrated male and females.
AB - The effect of luteinizing hormonereleasing hormone (LH-RH) on pituitary cytology was investigated in intact, hemicastrated, and castrated male and female rats using electron and light microscopy. Two hundred ng of LH-RH were injected into the carotid artery, and the animals were sacrificed 1, 3, and 9 min after the injection. Study of LH-containing gonadotrophs by an immunocytochemical method using peroxidase- labeled antibody showed depletion of peroxidase reactivity of LH cells 1 min after injection of LH-RH in intact and hemicastrated proestrous rats, and after 9 min in the castrated females. Serum LH levels measured by radioimmunoassay were found to be significantly elevated 3 and 9 min after LH-RH injection in intact, hemicastrated and castrated male and female rats. Serum FSH levels had risen 1, 3, and 9 min after injection of LH-RH in the intact males and hemicastrated proestrous females, had fallen 1 and 3 min after injection in the hemicastrated male and had not changed 1, 3, or 9 min after injection in the castrated male and females.
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U2 - 10.1210/endo-92-4-1153
DO - 10.1210/endo-92-4-1153
M3 - Article
C2 - 4568592
AN - SCOPUS:0015610049
VL - 92
SP - 1153
EP - 1160
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 4
ER -