Histological and immunohistological studies were performed on the ultimobranchial gland (UBG) of the trout, Salmo gairdneri. Histological studies of the UBG from these fish revealed that the gland is usually cystic. Peroxidase labeled antibodies used in an indirect immunohistological procedure showed that the hormone was primarily located in the epithelium and not in the cystic contents. Radioimmunoassay of dissected specimens indicated that 1-3% of the glandular protein was calcitonin. When the gland was fragmented by trypsinization cellular fractions could be obtained which contained 4-5% of their protein as calcitonin. These studies demonstrate the unique histological features of the trout UBG and show it to be a calcitonin-rich organ.
ASJC Scopus subject areas
- Animal Science and Zoology