Ubiquitinization of dystrophic mouse skeletal muscle proteins: Effect of IGF-I. M.M

Martin Zdanowicz, Wingertzahn

Research output: Contribution to journalArticle

Abstract

Ubiquitin (UB) is an 8.6 IcDa peptide involved in targeting of cellular proteins for degradation by an ATP-dependent degrading enzyme complex. Ubiquitinization of skeletal muscle proteins is increased with muscle injury Using Western blot we examined the Ubiquitinization of skeletal muscle proteins in 60 day old dystrophic (dy) 129 ReJ mice Dystrophic muscle showed greater levels of UB conjugated proteins (CUB) in the 10-50 kDa range and higher levels of free Ub (FUB) when compared to normal muscle (NORM). When dy animals were treated with rhIGF-I for 30 days (10 ug/day s.c.), the total amount of CUB and FUB was increased compared to NORM CUB CUB CUB FUB Group (10-50 kDa) f50-205 kOal (total) (total) dy 4.6±0.531.6±2.9 35.9±3.1 201±0.8IGFdy 5.5±0.539.9±3.9 45.3±4.325.3±05NORM 1.7±0.3 28,6±2.0 30.1±2.3 15,1±1.3 (values are units/50 ug protein,p<0.05,p<0.01 vs NORM, n=5 per group.) In summary, dystrophic muscle exhibits increased ubiqutinization of selected proteins along with increased levels of FUB. IGF-I increases Ubiquitinization of a number of muscle proteins while increasing levels of FUB Increased Ubiquitinization of proteins with IGF-I may be involved in the increased turnover of muscle proteins we observed in dy mice treated with IGF-I-.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

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Muscle Proteins
muscle protein
insulin-like growth factor I
Insulin-Like Growth Factor I
Muscle
skeletal muscle
Skeletal Muscle
Muscles
muscles
mice
ubiquitin
Ubiquitin
Proteins
conjugated proteins
129 Strain Mouse
proteins
protein degradation
Proteolysis
Animals
Western blotting

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Ubiquitinization of dystrophic mouse skeletal muscle proteins : Effect of IGF-I. M.M. / Zdanowicz, Martin; Wingertzahn.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

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