Tyrosine phosphorylation of guanosine triphosphatase activating protein by activation via surface IgG in human B cells

H. Yagura, N. Oyaizu, S. Pahwa

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

In this study, we analyzed tyrosine phosphorylation of guanosine triphosphatase (GTPase) activating protein in human B cells stimulated through surface IgG, using Western blot and immunoprecipitation. Stimulation through surface IgG induced the tyrosine phosphorylation of GTPase-activating protein (GAP) and two associated proteins, a 190-Kd protein and a 62-Kd protein, within 1 minute and in a dose-dependent manner. This tyrosine phosphorylation was blocked by Genistein (Extrasynthese, Genay, France). These data suggest that GTPase-activating protein is involved in a signal transduction pathway initiated from surface IgG in human B cells.

Original languageEnglish (US)
Pages (from-to)1535-1539
Number of pages5
JournalBlood
Volume81
Issue number6
DOIs
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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