Two structurally and functionally different forms of the transforming protein of PRC II avian sarcoma virus

Rebecca D Adkins, T. Hunter

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The primary translation product of the PRC II avian sarcoma virus genome is a protein of 105,000 daltons (P105), and we have previously shown that approximately 50% of the P105 molecules are converted to molecules of 110,000 daltons (P110) by posttranslational modification. Fractionation of PRC II-infected cells showed that P105 was contained primarily in a nonionic detergent-soluble compartment, whereas P110 partitioned almost exclusively with a nonionic detergent-insoluble or crude cytoskeletal fraction. The tyrosine-specific protein kinase activity previously observed in immunoprecipitates which presumably contained both P110 and P105 was found predominantly in the P110-containing immunoprecipitates made from the cytoskeletal fraction and was essentially absent from the P105-containing immunoprecipitates prepared from the soluble fraction. Individual analysis of 32P-labeled P110 and P105 prepared by this fractionation technique revelaed that P110 contained more phosphotyrosine per mole of protein than did P105. Examination of the tryptic peptide maps of 32P-labeled P110 and P105 suggested that the additional phosphotyrosine in P110 resulted from phosphorylation at discrete sites within the protein. From these experiments, we conclude that PRC II-infected cells contain two discrete forms, P105 and P110, of the transforming protein and that each of these proteins exhibits distinct structural and functional characteristics.

Original languageEnglish
Pages (from-to)890-896
Number of pages7
JournalMolecular and Cellular Biology
Volume2
Issue number8
StatePublished - Dec 1 1982
Externally publishedYes

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Avian Sarcoma Viruses
Proteins
Phosphotyrosine
Detergents
Post Translational Protein Processing

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Two structurally and functionally different forms of the transforming protein of PRC II avian sarcoma virus. / Adkins, Rebecca D; Hunter, T.

In: Molecular and Cellular Biology, Vol. 2, No. 8, 01.12.1982, p. 890-896.

Research output: Contribution to journalArticle

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