Two multidrug-resistant friend leukemic cell lines selected with different drugs exhibit overproduction of different p-glycoproteins

Niramol Savaraj, T. J. Lampidis, Ji Ying Zhao, Chun Jing Wu, Larry D. Teeter, M. Tien Kuo

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Two Friend leukemic multidrug-resistance (MDR) cell lines were established by exposure to stepwise increased concentrations of rhodumine-123 (RHO) (cell line RR-30) or Adriamycin (ADR) (cell line ARN-15). RR-30 displays preferential resistance to RHO, whereas ARN-15 is more resistant to ADR. The levels of resistance to other MDR drugs and reversibility by verapamil between these two MDR cell lines were somewhat different. Southern blot, RNase protection, and Western blot analysis using gene-specific probes demonstrated that RR-30 and ARN-15 cells preferentially amplified the mdrl and mdr3 genes. respectively, leading to overexpression of the corresponding P-glycoproteins (p-gp). Our results suggest that members of the mdr gene family can be amplified independently by using different selecting agents, which could be responsible for the differences in the sensitivities to these selecting agents as well as to these MDR drugs.

Original languageEnglish (US)
Pages (from-to)138-144
Number of pages7
JournalCancer Investigation
Volume12
Issue number2
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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