Two distinct lymphocyte populations mediate simian immunodeficiency virus envelope-specific target cell lysis

Hiroshi Yamamoto, Michael D. Miller, David Watkins, Gregory B. Snyder, Nadine E. Chase, Gail P. Mazzara, Linda Gritz, Dennis L. Panicali, Norman L. Letvin

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The relative contributions of the effector lymphocyte responses to the AIDS virus envelope glycoprotein (env) were explored in simian immunodeficiency virus of macaques (SIVmac)-infected rhesus monkeys. CD8+, MHC class I-restricted, env-specific CTL were cloned from PBL of SIVmac-infected monkeys, indicating that such cells constitute a component of the env-specific effector lymphocyte response. A limiting dilution 51Cr release assay was then established for quantitating the frequency of SIVmac-specific effector lymphocytes in PBL of rhesus monkeys. Using this assay we demonstrate that SIVmac env-specific effector lymphocytes are comprised of both CD16-, MHC class I-restricted and CD16+, MHC class I-unrestricted cells. We also demonstrate that the env-specific response is the predominant SIVmac-specific effector lymphocyte response in rhesus monkeys. These studies document the complexity of the effector lymphocyte response to the AIDS virus envelope glycoprotein and establish the role played by two distinct effector cell populations in this response.

Original languageEnglish
Pages (from-to)3740-3746
Number of pages7
JournalJournal of Immunology
Volume145
Issue number11
StatePublished - Dec 1 1990
Externally publishedYes

Fingerprint

Simian Immunodeficiency Virus
Macaca
Lymphocytes
Macaca mulatta
Population
HIV
env Gene Products
Haplorhini
Glycoproteins

ASJC Scopus subject areas

  • Immunology

Cite this

Yamamoto, H., Miller, M. D., Watkins, D., Snyder, G. B., Chase, N. E., Mazzara, G. P., ... Letvin, N. L. (1990). Two distinct lymphocyte populations mediate simian immunodeficiency virus envelope-specific target cell lysis. Journal of Immunology, 145(11), 3740-3746.

Two distinct lymphocyte populations mediate simian immunodeficiency virus envelope-specific target cell lysis. / Yamamoto, Hiroshi; Miller, Michael D.; Watkins, David; Snyder, Gregory B.; Chase, Nadine E.; Mazzara, Gail P.; Gritz, Linda; Panicali, Dennis L.; Letvin, Norman L.

In: Journal of Immunology, Vol. 145, No. 11, 01.12.1990, p. 3740-3746.

Research output: Contribution to journalArticle

Yamamoto, H, Miller, MD, Watkins, D, Snyder, GB, Chase, NE, Mazzara, GP, Gritz, L, Panicali, DL & Letvin, NL 1990, 'Two distinct lymphocyte populations mediate simian immunodeficiency virus envelope-specific target cell lysis', Journal of Immunology, vol. 145, no. 11, pp. 3740-3746.
Yamamoto H, Miller MD, Watkins D, Snyder GB, Chase NE, Mazzara GP et al. Two distinct lymphocyte populations mediate simian immunodeficiency virus envelope-specific target cell lysis. Journal of Immunology. 1990 Dec 1;145(11):3740-3746.
Yamamoto, Hiroshi ; Miller, Michael D. ; Watkins, David ; Snyder, Gregory B. ; Chase, Nadine E. ; Mazzara, Gail P. ; Gritz, Linda ; Panicali, Dennis L. ; Letvin, Norman L. / Two distinct lymphocyte populations mediate simian immunodeficiency virus envelope-specific target cell lysis. In: Journal of Immunology. 1990 ; Vol. 145, No. 11. pp. 3740-3746.
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