Two-color immunofluorescence flow cytometric analysis of lymphocytes in long-term renal allograft recipients

M. Ziegelbaum, R. Valenzuela, J. P. Hayes, D. Steinmuller, S. B. Streem, A. C. Novick

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We used 2-color immunofluorescence flow cytometry (FACS 440) and monoclonal antibodies (Becton-Dickinson) to study peripheral blood lymphocyte subsets from 26 patients with well functioning renal allografts for longer than 5 years. Comparisons were made to a group of 25 healthy volunteers and 25 dialysis patients awaiting renal transplantation. As anticipated, there was no significant difference with respect to the absolute number of percentage of activated (T11+ and HLA-DR+) and cytotoxic (Leu-2+ and Leu-15-) T lymphocytes among the 3 groups. However, there was a significantly decreased absolute number (p equals 0.0001) and percentage (p equals 0.0001) of suppressor cells (Leu-2+ and Leu-15+) in the transplant patients compared to the healthy control group. No significant difference existed between the transplant and dialysis groups. There also was an increase in the percentage (p equals 0.002) but not in the absolute number of T helper lymphocytes (Leu-3+ and Leu-8-) in the transplant population compared to healthy controls. No significant difference existed between the transplant and dialysis groups. These findings suggest that a normal value of cytotoxic (Leu-2+ and Leu-15-) and activated (T11+ and HLA-Dr+) T lymphocytes may be a good prognostic indicator of long-term survival of renal allografts. Also, the highly significant decrease in the number of Leu-2+ and Leu-15+ cells in long-term, well functioning allografts indicates that additional functional characterization of this subset may be necessary.

Original languageEnglish
Pages (from-to)492-494
Number of pages3
JournalJournal of Urology
Volume141
Issue number3 I
StatePublished - Jan 1 1989
Externally publishedYes

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Fluorescent Antibody Technique
Allografts
Color
Lymphocytes
Transplants
Kidney
Dialysis
T-Lymphocytes
Lymphocyte Subsets
HLA-DR Antigens
Helper-Inducer T-Lymphocytes
Kidney Transplantation
Healthy Volunteers
Flow Cytometry
Reference Values
Monoclonal Antibodies
Control Groups
Population

ASJC Scopus subject areas

  • Urology

Cite this

Ziegelbaum, M., Valenzuela, R., Hayes, J. P., Steinmuller, D., Streem, S. B., & Novick, A. C. (1989). Two-color immunofluorescence flow cytometric analysis of lymphocytes in long-term renal allograft recipients. Journal of Urology, 141(3 I), 492-494.

Two-color immunofluorescence flow cytometric analysis of lymphocytes in long-term renal allograft recipients. / Ziegelbaum, M.; Valenzuela, R.; Hayes, J. P.; Steinmuller, D.; Streem, S. B.; Novick, A. C.

In: Journal of Urology, Vol. 141, No. 3 I, 01.01.1989, p. 492-494.

Research output: Contribution to journalArticle

Ziegelbaum, M, Valenzuela, R, Hayes, JP, Steinmuller, D, Streem, SB & Novick, AC 1989, 'Two-color immunofluorescence flow cytometric analysis of lymphocytes in long-term renal allograft recipients', Journal of Urology, vol. 141, no. 3 I, pp. 492-494.
Ziegelbaum M, Valenzuela R, Hayes JP, Steinmuller D, Streem SB, Novick AC. Two-color immunofluorescence flow cytometric analysis of lymphocytes in long-term renal allograft recipients. Journal of Urology. 1989 Jan 1;141(3 I):492-494.
Ziegelbaum, M. ; Valenzuela, R. ; Hayes, J. P. ; Steinmuller, D. ; Streem, S. B. ; Novick, A. C. / Two-color immunofluorescence flow cytometric analysis of lymphocytes in long-term renal allograft recipients. In: Journal of Urology. 1989 ; Vol. 141, No. 3 I. pp. 492-494.
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