Two and three dimensional metabolite mapping of cerebral metabolites in rat by magnetic resonance spectroscopic imaging

E. J. Fernandex, T. Higuchi, A. A. Maudsley, M. W. Weiner

Research output: Contribution to journalConference articlepeer-review


Recently, spectroscopic imaging (SI) techniques based on phase encoding have made it possible to obtain metabolite images using 31P and 1H magnetic resonance (MR). These techniques are useful in studies of spatially heterogeneous metabolism, for example, cerebral metabolism during stroke. However, 1H MR SI development has been slowed by the obstacles of sensitivity and water and lipid suppression. We have developed spin-echo 1H MR SI pulse sequences for monitoring metabolite distributions in two and three dimensions. Metabolites observed include choline, creatine, amino acids, N-acetyl asparate, and lactate. A birdcage volume coil was used to obtain homogeneous two dimensional metabolite images of the brain and the entire rat head (3 mm × 3 mm in plane resolution, 7 mm slice). Subsequently, the greater sensitivity of surface coils was exploited to obtain three dimensional metabolite images of higher resolution (2 mm × 2.5 mm × 2 mm voxel size) than with the volume coil in the same acquisition time (45-60 minutes). Adiabatic excitation and refocussing pulses were employed to circumvent the heterogeneous rf transmission characteristics of surface coils. Volume and surface coil SI acquisitions were compared in terms of sensitivity, water suppression efficiency, and spectral resolution for the acquisition of SI data sets in two or three spatial dimensions. Results will be presented from studies of rat brain under normal and focal ischemia conditions. The image and data processing challenges presented by this application will be discussed.

Original languageEnglish (US)
Pages (from-to)614-615
Number of pages2
JournalAnnals of biomedical engineering
Issue number5
StatePublished - Dec 1 1991
Externally publishedYes
Event1991 Annual Fall Meeting of the Biomedical Engineering Society - Charlottesville, VA, USA
Duration: Oct 12 1991Oct 14 1991

ASJC Scopus subject areas

  • Biomedical Engineering


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