Turn-on, fluorescent nuclear stains with live cell compatibility

Demar R.G. Pitter; Jens Wigenius; Adrienne S. Brown; James D. Baker; Fredrik Westerlund; James N. Wilson

doi:10.1021/ol400268t

Turn-on, fluorescent nuclear stains with live cell compatibility

Demar R.G. Pitter, Jens Wigenius, Adrienne S. Brown, James D. Baker, Fredrik Westerlund, James N. Wilson

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

Original language	English (US)
Pages (from-to)	1330-1333
Number of pages	4
Journal	Organic letters
Volume	15
Issue number	6
DOIs	https://doi.org/10.1021/ol400268t
State	Published - Mar 15 2013

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

Access to Document

10.1021/ol400268t

Fingerprint Dive into the research topics of 'Turn-on, fluorescent nuclear stains with live cell compatibility'. Together they form a unique fingerprint.

Cite this

@article{3ceeb7963cda43d09f659d9185b0f2df,

title = "Turn-on, fluorescent nuclear stains with live cell compatibility",

abstract = "DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.",

author = "Pitter, {Demar R.G.} and Jens Wigenius and Brown, {Adrienne S.} and Baker, {James D.} and Fredrik Westerlund and Wilson, {James N.}",

year = "2013",

month = mar,

day = "15",

doi = "10.1021/ol400268t",

language = "English (US)",

volume = "15",

pages = "1330--1333",

journal = "Organic Letters",

issn = "1523-7060",

publisher = "American Chemical Society",

number = "6",

}

TY - JOUR

T1 - Turn-on, fluorescent nuclear stains with live cell compatibility

AU - Pitter, Demar R.G.

AU - Wigenius, Jens

AU - Brown, Adrienne S.

AU - Baker, James D.

AU - Westerlund, Fredrik

AU - Wilson, James N.

PY - 2013/3/15

Y1 - 2013/3/15

N2 - DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

AB - DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

UR - http://www.scopus.com/inward/record.url?scp=84875164849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875164849&partnerID=8YFLogxK

U2 - 10.1021/ol400268t

DO - 10.1021/ol400268t

M3 - Article

C2 - 23461418

AN - SCOPUS:84875164849

VL - 15

SP - 1330

EP - 1333

JO - Organic Letters

JF - Organic Letters

SN - 1523-7060

IS - 6

ER -

Turn-on, fluorescent nuclear stains with live cell compatibility

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this