Turn-on, fluorescent nuclear stains with live cell compatibility

Demar R.G. Pitter; Jens Wigenius; Adrienne S. Brown; James D. Baker; Fredrik Westerlund; James N. Wilson

doi:10.1021/ol400268t

Turn-on, fluorescent nuclear stains with live cell compatibility

Demar R.G. Pitter, Jens Wigenius, Adrienne S. Brown, James D. Baker, Fredrik Westerlund, James N. Wilson

Research output: Contribution to journal › Article

25 Scopus citations

Abstract

DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

Original language	English (US)
Pages (from-to)	1330-1333
Number of pages	4
Journal	Organic letters
Volume	15
Issue number	6
DOIs	https://doi.org/10.1021/ol400268t
State	Published - Mar 15 2013

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol400268t

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Pitter, D. R. G., Wigenius, J., Brown, A. S., Baker, J. D., Westerlund, F., & Wilson, J. N. (2013). Turn-on, fluorescent nuclear stains with live cell compatibility. Organic letters, 15(6), 1330-1333. https://doi.org/10.1021/ol400268t

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AU - Wigenius, Jens

AU - Brown, Adrienne S.

AU - Baker, James D.

AU - Westerlund, Fredrik

AU - Wilson, James N.

PY - 2013/3/15

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AB - DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

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