Turn-on, fluorescent nuclear stains with live cell compatibility

Demar R.G. Pitter, Jens Wigenius, Adrienne S. Brown, James D. Baker, Fredrik Westerlund, James N. Wilson

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

Original languageEnglish (US)
Pages (from-to)1330-1333
Number of pages4
JournalOrganic letters
Volume15
Issue number6
DOIs
StatePublished - Mar 15 2013

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ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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