Abstract
DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 1330-1333 |
| Number of pages | 4 |
| Journal | Organic Letters |
| Volume | 15 |
| Issue number | 6 |
| DOIs | |
| State | Published - Mar 15 2013 |
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ASJC Scopus subject areas
- Organic Chemistry
- Physical and Theoretical Chemistry
- Biochemistry
Cite this
Turn-on, fluorescent nuclear stains with live cell compatibility. / Pitter, Demar R G; Wigenius, Jens; Brown, Adrienne S.; Baker, James; Westerlund, Fredrik; Wilson, James N.
In: Organic Letters, Vol. 15, No. 6, 15.03.2013, p. 1330-1333.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Turn-on, fluorescent nuclear stains with live cell compatibility
AU - Pitter, Demar R G
AU - Wigenius, Jens
AU - Brown, Adrienne S.
AU - Baker, James
AU - Westerlund, Fredrik
AU - Wilson, James N
PY - 2013/3/15
Y1 - 2013/3/15
N2 - DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.
AB - DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.
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U2 - 10.1021/ol400268t
DO - 10.1021/ol400268t
M3 - Article
VL - 15
SP - 1330
EP - 1333
JO - Organic Letters
JF - Organic Letters
SN - 1523-7060
IS - 6
ER -