Turn-on, fluorescent nuclear stains with live cell compatibility

Demar R G Pitter, Jens Wigenius, Adrienne S. Brown, James Baker, Fredrik Westerlund, James N Wilson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

DNA-binding, green and yellow fluorescent probes with excellent brightness and high on/off ratios are reported. The probes are membrane permeable, live-cell compatible, and optimally matched to 405 nm and 514 nm laser lines, making them attractive alternatives to UV-excited and blue emissive Hoechst 33342 and DAPI nuclear stains. Their electronic structure was investigated by optical spectroscopy supported by TD-DFT calculations. DNA binding is accompanied by 27- to 75-fold emission enhancements, and linear dichroism demonstrates that one dye is a groove binder while the other intercalates into DNA.

Original languageEnglish
Pages (from-to)1330-1333
Number of pages4
JournalOrganic Letters
Volume15
Issue number6
DOIs
StatePublished - Mar 15 2013

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compatibility
Coloring Agents
deoxyribonucleic acid
DNA
cells
probes
Fluorescent Dyes
Discrete Fourier transforms
grooves
dichroism
Electronic structure
Binders
Luminance
Spectrum Analysis
brightness
Lasers
dyes
electronic structure
membranes
Membranes

ASJC Scopus subject areas

  • Organic Chemistry
  • Physical and Theoretical Chemistry
  • Biochemistry

Cite this

Turn-on, fluorescent nuclear stains with live cell compatibility. / Pitter, Demar R G; Wigenius, Jens; Brown, Adrienne S.; Baker, James; Westerlund, Fredrik; Wilson, James N.

In: Organic Letters, Vol. 15, No. 6, 15.03.2013, p. 1330-1333.

Research output: Contribution to journalArticle

Pitter, DRG, Wigenius, J, Brown, AS, Baker, J, Westerlund, F & Wilson, JN 2013, 'Turn-on, fluorescent nuclear stains with live cell compatibility', Organic Letters, vol. 15, no. 6, pp. 1330-1333. https://doi.org/10.1021/ol400268t
Pitter, Demar R G ; Wigenius, Jens ; Brown, Adrienne S. ; Baker, James ; Westerlund, Fredrik ; Wilson, James N. / Turn-on, fluorescent nuclear stains with live cell compatibility. In: Organic Letters. 2013 ; Vol. 15, No. 6. pp. 1330-1333.
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