Tumour Necrosis Factor Alpha, Interferon Gamma and Substance P Are Novel Modulators of Extrapituitary Prolactin Expression in Human Skin

Ewan A. Langan, Silvia Vidali, Natascha Pigat, Wolfgang Funk, Erika Lisztes, Tamás Bíró, Vincent Goffin, Christopher E.M. Griffiths, Ralf Paus

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Human scalp skin and hair follicles (HFs) are extra-pituitary sources of prolactin (PRL). However, the intracutaneous regulation of PRL remains poorly understood. Therefore we investigated whether well-recognized regulators of pituitary PRL expression, which also impact on human skin physiology and pathology, regulate expression of PRL and its receptor (PRLR) in situ. This was studied in serum-free organ cultures of microdissected human scalp HFs and skin, i.e. excluding pituitary, neural and vascular inputs. Prolactin expression was confirmed at the gene and protein level in human truncal skin, where its expression significantly increased (p = 0.049) during organ culture. There was, however, no evidence of PRL secretion into the culture medium as measured by ELISA. PRL immunoreactivity (IR) in female human epidermis was decreased by substance P (p = 0.009), while neither the classical pituitary PRL inhibitor, dopamine, nor corticotropin-releasing hormone significantly modulated PRL IR in HFs or skin respectively. Interferon (IFN) γ increased PRL IR in the epithelium of human HFs (p = 0.044) while tumour necrosis factor (TNF) α decreased both PRL and PRLR IR. This study identifies substance P, TNFα and IFNγ as novel modulators of PRL and PRLR expression in human skin, and suggests that intracutaneous PRL expression is not under dopaminergic control. Given the importance of PRL in human hair growth regulation and its possible role in the pathogenesis of several common skin diseases, targeting intracutaneous PRL production via these newly identified regulatory pathways may point towards novel therapeutic options for inflammatory dermatoses.

Original languageEnglish (US)
Article numbere60819
JournalPLoS One
Volume8
Issue number4
DOIs
StatePublished - Apr 23 2013
Externally publishedYes

Fingerprint

interferon-alpha
substance P
Substance P
prolactin
interferon-gamma
Interferon-alpha
skin (animal)
Prolactin
Modulators
tumor necrosis factor-alpha
Interferon-gamma
Skin
Tumor Necrosis Factor-alpha
Hair Follicle
hair follicles
Prolactin Receptors
Organ Culture Techniques
organ culture
tumor necrosis factors
skin diseases

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tumour Necrosis Factor Alpha, Interferon Gamma and Substance P Are Novel Modulators of Extrapituitary Prolactin Expression in Human Skin. / Langan, Ewan A.; Vidali, Silvia; Pigat, Natascha; Funk, Wolfgang; Lisztes, Erika; Bíró, Tamás; Goffin, Vincent; Griffiths, Christopher E.M.; Paus, Ralf.

In: PLoS One, Vol. 8, No. 4, e60819, 23.04.2013.

Research output: Contribution to journalArticle

Langan, Ewan A. ; Vidali, Silvia ; Pigat, Natascha ; Funk, Wolfgang ; Lisztes, Erika ; Bíró, Tamás ; Goffin, Vincent ; Griffiths, Christopher E.M. ; Paus, Ralf. / Tumour Necrosis Factor Alpha, Interferon Gamma and Substance P Are Novel Modulators of Extrapituitary Prolactin Expression in Human Skin. In: PLoS One. 2013 ; Vol. 8, No. 4.
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