Tumor necrosis factor-alpha in plasma during cardiopulmonary bypass in a pig model

Correlation with marginated neutrophils and cerebral edema by magnetic resonance imaging

Mrinal K. Dewanjee, Shu Ming Wu, George W Burke, Li Chien Hsu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Tumor necrosis factor (TNFα) initiates the cytokine cascade via the nuclear factor (NFκB), increasing vascular permeability, inflammation, and edema during and after cardiopulmonary bypass (CPB). Cerebral edema before and after thoracotomy and CPB was evaluated by magnetic resonance imaging. TNF-α in plasma was measured in 12 Yorkshire pigs with an enzyme linked immunosorbent assay technique using a monoclonal antibody made against porcine TNFα. Blood samples were taken 30 min before and 1, 30, 60, 90, 120, 150, and 180 min after initiation of CPB. Plasma was separated from packed cells after centrifugation, and frozen at -80°C. The level of TNFα, measured by color intensity, was read with a 96 well microtiter plate reader. Normal values in plasma were low (range, 0-17 pg/ml; mean, 4.7 pg/ml). In sham control pigs undergoing sternotomy, and pigs that had undergone CBP, TNFα reached a peak value at 120-150 min, and subsequently declined. TNFα in plasma increased in pigs that underwent thoracotomy and CPB. The increase was significantly higher in pigs that underwent CPB. Neutrophil mapping in the pig brain with 111In-labeled autologous neutrophils 180 min after CPB, suggested almost uniform distribution and accounted for the 0.02% of total circulating neutrophils in the brain, corresponding to a total of 8 x 105 neutrophils. Magnetic resonance imaging of the pig brain indicated the presence of cerebral edema after CPB, as depicted by the loss of structural details of the sulci, gyri, and ventricles. Activated neutrophils, monocytes, and other inflammatory cells may induce multi-organ edema and injury via TNFα and other regional cytokines.

Original languageEnglish
Pages (from-to)212-218
Number of pages7
JournalASAIO Journal
Volume44
Issue number3
StatePublished - Jun 1 1998
Externally publishedYes

Fingerprint

Brain Edema
Magnetic resonance
Cardiopulmonary Bypass
Neutrophils
Swine
Tumor Necrosis Factor-alpha
Magnetic Resonance Imaging
Plasmas
Imaging techniques
Brain
Thoracotomy
Edema
Cytokines
Immunosorbent Techniques
Immunosorbents
Monoclonal antibodies
Centrifugation
Sternotomy
Capillary Permeability
Magnetic resonance imaging

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Tumor necrosis factor-alpha in plasma during cardiopulmonary bypass in a pig model : Correlation with marginated neutrophils and cerebral edema by magnetic resonance imaging. / Dewanjee, Mrinal K.; Wu, Shu Ming; Burke, George W; Hsu, Li Chien.

In: ASAIO Journal, Vol. 44, No. 3, 01.06.1998, p. 212-218.

Research output: Contribution to journalArticle

@article{5510e7238fda4e51a5e6395297d51438,
title = "Tumor necrosis factor-alpha in plasma during cardiopulmonary bypass in a pig model: Correlation with marginated neutrophils and cerebral edema by magnetic resonance imaging",
abstract = "Tumor necrosis factor (TNFα) initiates the cytokine cascade via the nuclear factor (NFκB), increasing vascular permeability, inflammation, and edema during and after cardiopulmonary bypass (CPB). Cerebral edema before and after thoracotomy and CPB was evaluated by magnetic resonance imaging. TNF-α in plasma was measured in 12 Yorkshire pigs with an enzyme linked immunosorbent assay technique using a monoclonal antibody made against porcine TNFα. Blood samples were taken 30 min before and 1, 30, 60, 90, 120, 150, and 180 min after initiation of CPB. Plasma was separated from packed cells after centrifugation, and frozen at -80°C. The level of TNFα, measured by color intensity, was read with a 96 well microtiter plate reader. Normal values in plasma were low (range, 0-17 pg/ml; mean, 4.7 pg/ml). In sham control pigs undergoing sternotomy, and pigs that had undergone CBP, TNFα reached a peak value at 120-150 min, and subsequently declined. TNFα in plasma increased in pigs that underwent thoracotomy and CPB. The increase was significantly higher in pigs that underwent CPB. Neutrophil mapping in the pig brain with 111In-labeled autologous neutrophils 180 min after CPB, suggested almost uniform distribution and accounted for the 0.02{\%} of total circulating neutrophils in the brain, corresponding to a total of 8 x 105 neutrophils. Magnetic resonance imaging of the pig brain indicated the presence of cerebral edema after CPB, as depicted by the loss of structural details of the sulci, gyri, and ventricles. Activated neutrophils, monocytes, and other inflammatory cells may induce multi-organ edema and injury via TNFα and other regional cytokines.",
author = "Dewanjee, {Mrinal K.} and Wu, {Shu Ming} and Burke, {George W} and Hsu, {Li Chien}",
year = "1998",
month = "6",
day = "1",
language = "English",
volume = "44",
pages = "212--218",
journal = "ASAIO Journal",
issn = "1058-2916",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Tumor necrosis factor-alpha in plasma during cardiopulmonary bypass in a pig model

T2 - Correlation with marginated neutrophils and cerebral edema by magnetic resonance imaging

AU - Dewanjee, Mrinal K.

AU - Wu, Shu Ming

AU - Burke, George W

AU - Hsu, Li Chien

PY - 1998/6/1

Y1 - 1998/6/1

N2 - Tumor necrosis factor (TNFα) initiates the cytokine cascade via the nuclear factor (NFκB), increasing vascular permeability, inflammation, and edema during and after cardiopulmonary bypass (CPB). Cerebral edema before and after thoracotomy and CPB was evaluated by magnetic resonance imaging. TNF-α in plasma was measured in 12 Yorkshire pigs with an enzyme linked immunosorbent assay technique using a monoclonal antibody made against porcine TNFα. Blood samples were taken 30 min before and 1, 30, 60, 90, 120, 150, and 180 min after initiation of CPB. Plasma was separated from packed cells after centrifugation, and frozen at -80°C. The level of TNFα, measured by color intensity, was read with a 96 well microtiter plate reader. Normal values in plasma were low (range, 0-17 pg/ml; mean, 4.7 pg/ml). In sham control pigs undergoing sternotomy, and pigs that had undergone CBP, TNFα reached a peak value at 120-150 min, and subsequently declined. TNFα in plasma increased in pigs that underwent thoracotomy and CPB. The increase was significantly higher in pigs that underwent CPB. Neutrophil mapping in the pig brain with 111In-labeled autologous neutrophils 180 min after CPB, suggested almost uniform distribution and accounted for the 0.02% of total circulating neutrophils in the brain, corresponding to a total of 8 x 105 neutrophils. Magnetic resonance imaging of the pig brain indicated the presence of cerebral edema after CPB, as depicted by the loss of structural details of the sulci, gyri, and ventricles. Activated neutrophils, monocytes, and other inflammatory cells may induce multi-organ edema and injury via TNFα and other regional cytokines.

AB - Tumor necrosis factor (TNFα) initiates the cytokine cascade via the nuclear factor (NFκB), increasing vascular permeability, inflammation, and edema during and after cardiopulmonary bypass (CPB). Cerebral edema before and after thoracotomy and CPB was evaluated by magnetic resonance imaging. TNF-α in plasma was measured in 12 Yorkshire pigs with an enzyme linked immunosorbent assay technique using a monoclonal antibody made against porcine TNFα. Blood samples were taken 30 min before and 1, 30, 60, 90, 120, 150, and 180 min after initiation of CPB. Plasma was separated from packed cells after centrifugation, and frozen at -80°C. The level of TNFα, measured by color intensity, was read with a 96 well microtiter plate reader. Normal values in plasma were low (range, 0-17 pg/ml; mean, 4.7 pg/ml). In sham control pigs undergoing sternotomy, and pigs that had undergone CBP, TNFα reached a peak value at 120-150 min, and subsequently declined. TNFα in plasma increased in pigs that underwent thoracotomy and CPB. The increase was significantly higher in pigs that underwent CPB. Neutrophil mapping in the pig brain with 111In-labeled autologous neutrophils 180 min after CPB, suggested almost uniform distribution and accounted for the 0.02% of total circulating neutrophils in the brain, corresponding to a total of 8 x 105 neutrophils. Magnetic resonance imaging of the pig brain indicated the presence of cerebral edema after CPB, as depicted by the loss of structural details of the sulci, gyri, and ventricles. Activated neutrophils, monocytes, and other inflammatory cells may induce multi-organ edema and injury via TNFα and other regional cytokines.

UR - http://www.scopus.com/inward/record.url?scp=0345055829&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345055829&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 212

EP - 218

JO - ASAIO Journal

JF - ASAIO Journal

SN - 1058-2916

IS - 3

ER -