Tumor microenvironment profoundly modifies functional status of macrophages: Peritoneal and tumor-associated macrophages are two very different subpopulations

Dayron Rodríguez, Risset Silvera, Roberto Carrio, Mehrdad Nadji, Raul Caso, Gracielena Rodríguez, Vijaya Iragavarapu-Charyulu, Marta Torroella-Kouri

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Macrophages are key players in the inflammatory response. In this study, we tested the hypothesis that although all macrophage subpopulations in tumor hosts are affected by the disease, it is the close proximity to the tumor that induces major alterations in these cells. We compared tumor-associated macrophages (TAMs) with peritoneal macrophages from mice bearing D1-DMBA-3 mammary tumors (T-PEMs). Our results show that TAMs downregulate IL-12p70 but upregulate IL-12p40, IL-23, IL-6 and IL-10. Some NFκB and C/EBP transcription factors family members are decreased in TAMs; however NFκBp50 homodimers, STAT1/pSTAT1 and STAT3/pSTAT3 are overexpressed. Furthermore, while TAMs block T-cell proliferation and are more prone to apoptosis compared to T-PEMs, both types of macrophages have an impaired phagocytic capacity. Moreover, TAMs constitutively express iNOS and produce nitric oxide but do not express arginase and are Gr-1high and CD11blow. Collectively, our analysis of two spatially distinct macrophage subpopulations in tumor-bearing mice revealed that the tumor modulates them differently into two molecularly and functionally dissimilar macrophage subpopulations.

Original languageEnglish
Pages (from-to)51-60
Number of pages10
JournalCellular Immunology
Volume283
Issue number1-2
DOIs
StatePublished - May 1 2013

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Tumor Microenvironment
Peritoneal Macrophages
Macrophages
Neoplasms
Interleukin-12 Subunit p40
Interleukin-23
Arginase
9,10-Dimethyl-1,2-benzanthracene
Interleukin-10
Interleukin-6
Nitric Oxide
Transcription Factors
Up-Regulation
Down-Regulation
Cell Proliferation
Apoptosis
Breast Neoplasms
T-Lymphocytes

Keywords

  • Immunosuppression
  • Inflammation
  • Peritoneal and tumor-associated macrophages
  • Tumor microenvironment

ASJC Scopus subject areas

  • Immunology

Cite this

Tumor microenvironment profoundly modifies functional status of macrophages : Peritoneal and tumor-associated macrophages are two very different subpopulations. / Rodríguez, Dayron; Silvera, Risset; Carrio, Roberto; Nadji, Mehrdad; Caso, Raul; Rodríguez, Gracielena; Iragavarapu-Charyulu, Vijaya; Torroella-Kouri, Marta.

In: Cellular Immunology, Vol. 283, No. 1-2, 01.05.2013, p. 51-60.

Research output: Contribution to journalArticle

Rodríguez, Dayron ; Silvera, Risset ; Carrio, Roberto ; Nadji, Mehrdad ; Caso, Raul ; Rodríguez, Gracielena ; Iragavarapu-Charyulu, Vijaya ; Torroella-Kouri, Marta. / Tumor microenvironment profoundly modifies functional status of macrophages : Peritoneal and tumor-associated macrophages are two very different subpopulations. In: Cellular Immunology. 2013 ; Vol. 283, No. 1-2. pp. 51-60.
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