Tumor-associated antigen in human pancreatic cancer

D. R. Schultz, Adel A Yunis

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

New Zealand White rabbits were immunized with saline extracts of 1) a human pancreatic cancer cell line (MIA PaCa-2) 2) human pancreatic tumors excised from Swiss nu/nu mice, or 3) human pancreatic cancer tissue. Solid-phase immunoadsorbents rendered the resulting antisera specific. The antisera, tested by double immunodiffusion and counterimmunoelectrophoresis (CEP), detected the pancreatic antigen in saline extracts of human pancreatic carcinoma, human fetal pancreas, MIA PaCa-2 cells, a second human pancreatic cancer cell line (PANC-1), and nude mouse pancreatic tumors, but not in saline extracts of normal human pancreas and a number of other normal tissues, in normal human sera, or in sera from patients with active inflammatory disease. The antisera did not react with alpha-fetoprotein and carcinoembryonic antigen. Sera of patients with pancreatic cancer and other neoplastic and nonneoplastic disorders were tested in CEP with two antisera: rabbit anti-MIA PaCa-2 and rabbit anti-human pancreatic carcinoma. Although both antisera detected the pancreatic antigen in 65-70% of the patients with biopsy-confirmed pancreatic carcinoma, the antihuman pancreatic carcinoma serum was less reactive with sera from patients having disorders not involving the pancreas. Rabbit anti-MIA PaCa-2 serum added to 5-day-old washed and fixed MIA PaCa-2 cells, followed by Fluorescein-labeled goat antirabbit serum, resulted in strong fluorescence located on the nuclear membranes. Additional antigen was released from saline-extracted cell membranes after treatment with Triton X-100. The estimated molecular weight of the tumor-associated pancreatic antigen was between 900 x 103 and 1,000 x 103 daltons. The antigen migrated in the β1 to a2 regions in agarose electrophoresis and was destroyed by 10% percholic acid or by boiling.

Original languageEnglish
Pages (from-to)777-785
Number of pages9
JournalJournal of the National Cancer Institute
Volume62
Issue number4
StatePublished - Jan 1 1979

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Neoplasm Antigens
Pancreatic Neoplasms
Immune Sera
Serum
Antigens
Counterimmunoelectrophoresis
Rabbits
Pancreas
Cell Line
Neoplasms
Immunosorbents
Immunodiffusion
Carcinoembryonic Antigen
Nuclear Envelope
Octoxynol
alpha-Fetoproteins
Fluorescein
Goats
Nude Mice
Sepharose

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tumor-associated antigen in human pancreatic cancer. / Schultz, D. R.; Yunis, Adel A.

In: Journal of the National Cancer Institute, Vol. 62, No. 4, 01.01.1979, p. 777-785.

Research output: Contribution to journalArticle

Schultz, DR & Yunis, AA 1979, 'Tumor-associated antigen in human pancreatic cancer', Journal of the National Cancer Institute, vol. 62, no. 4, pp. 777-785.
Schultz, D. R. ; Yunis, Adel A. / Tumor-associated antigen in human pancreatic cancer. In: Journal of the National Cancer Institute. 1979 ; Vol. 62, No. 4. pp. 777-785.
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