TY - JOUR
T1 - Tumor antigen extracts stimulate cytotoxicity against unrelated target cells
AU - Lopez, Diana M.
AU - Sigel, M. Michael
PY - 1976/12/15
Y1 - 1976/12/15
N2 - A new cytotoxicity assay for the study of tumor antigens is described. Splenocytes from normal and mammary tumor bearing BALB/c mice were depleted of glass adherent cells, exposed to various concentrations of tumor extracts and mixed with 51Cr labelled chick red blood cells. Cytolysis evidenced by 51Cr release was determined 20 h after incubation at 37°C. Lymphocytes from tumor bearers exerted no cytotoxic action per se, but became cytolytic after confrontation with immunogenic tumor extracts. Although this cytotoxicity is expressed against targets other than the cells containing the sensitizing antigen, i.e., a non specific activity, its induction is strictly specific as evidenced by the following: (1) extracts of D1-DMBA 3 tumors exerted an inductive effect only in lymphocytes from mice bearing this tumor and not in lymphocytes from normal mice; and (2) extracts of normal tissues or of a non immunogenic mammary tumor, D1-DMBA 2, did not induce cytotoxicity in lymphocytes from normal or tumor bearing mice. This approach may provide a useful method for in vitro determination of cell mediated immunity in which specific reactions can be induced by tumor extracts as opposed to intact tumor cells.
AB - A new cytotoxicity assay for the study of tumor antigens is described. Splenocytes from normal and mammary tumor bearing BALB/c mice were depleted of glass adherent cells, exposed to various concentrations of tumor extracts and mixed with 51Cr labelled chick red blood cells. Cytolysis evidenced by 51Cr release was determined 20 h after incubation at 37°C. Lymphocytes from tumor bearers exerted no cytotoxic action per se, but became cytolytic after confrontation with immunogenic tumor extracts. Although this cytotoxicity is expressed against targets other than the cells containing the sensitizing antigen, i.e., a non specific activity, its induction is strictly specific as evidenced by the following: (1) extracts of D1-DMBA 3 tumors exerted an inductive effect only in lymphocytes from mice bearing this tumor and not in lymphocytes from normal mice; and (2) extracts of normal tissues or of a non immunogenic mammary tumor, D1-DMBA 2, did not induce cytotoxicity in lymphocytes from normal or tumor bearing mice. This approach may provide a useful method for in vitro determination of cell mediated immunity in which specific reactions can be induced by tumor extracts as opposed to intact tumor cells.
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U2 - 10.1002/ijc.2910180612
DO - 10.1002/ijc.2910180612
M3 - Article
C2 - 992908
AN - SCOPUS:0017114291
VL - 18
SP - 808
EP - 812
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 6
ER -