Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System

Elif Vardar, Eva Maria Engelhardt, Hans M. Larsson, Elodie Mouloungui, Kalitha Pinnagoda, Jeffrey A. Hubbell, Peter Frey

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Ureteral replacement by tissue engineering might become necessary following tissue loss after excessive ureteral trauma, after retroperitoneal cancer, or even after failed reconstructive surgery. This need has driven innovation in the design of novel scaffolds and specific cell culture techniques for urinary tract reconstruction. In this study, compressed tubular collagen scaffolds were evaluated, addressing the physical and biological characterization of acellular and cellular collagen tubes in a new flow bioreactor system, imitating the physiological pressure, peristalsis, and flow conditions of the human ureter. Collagen tubes, containing primary human smooth muscle and urothelial cells, were evaluated regarding their change in gene and protein expression under dynamic culture conditions. A maximum intraluminal pressure of 22.43±0.2cmH<inf>2</inf>O was observed in acellular tubes, resulting in a mean wall shear stress of 4 dynes/cm<sup>2</sup> in the tubular constructs. Dynamic conditions directed the differentiation of both cell types into their mature forms. This was confirmed by their gene expression of smooth muscle alpha-actin, smoothelin, collagen type I and III, elastin, laminin type 1 and 5, cytokeratin 8, and uroplakin 2. In addition, smooth muscle cell alignment predominantly perpendicular to the flow direction was observed, comparable to the cell orientation in native ureteral tissue. These results revealed that coculturing human smooth muscle and urothelial cells in compressed collagen tubes under human ureteral flow-mimicking conditions could lead to cell-engineered biomaterials that might ultimately be translated into clinical applications.

Original languageEnglish (US)
Pages (from-to)2334-2345
Number of pages12
JournalTissue Engineering - Part A
Volume21
Issue number17-18
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

Fingerprint

Bioreactors
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Collagen
Muscle
Smooth Muscle Myocytes
Uroplakin II
Keratin-2
Reconstructive Surgical Procedures
Cell culture
Tissue
Keratin-8
Gene Expression
Pressure
Peristalsis
Collagen Type III
Elastin
Biocompatible Materials
Ureter

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Vardar, E., Engelhardt, E. M., Larsson, H. M., Mouloungui, E., Pinnagoda, K., Hubbell, J. A., & Frey, P. (2015). Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System. Tissue Engineering - Part A, 21(17-18), 2334-2345. https://doi.org/10.1089/ten.tea.2015.0048

Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System. / Vardar, Elif; Engelhardt, Eva Maria; Larsson, Hans M.; Mouloungui, Elodie; Pinnagoda, Kalitha; Hubbell, Jeffrey A.; Frey, Peter.

In: Tissue Engineering - Part A, Vol. 21, No. 17-18, 01.09.2015, p. 2334-2345.

Research output: Contribution to journalArticle

Vardar, E, Engelhardt, EM, Larsson, HM, Mouloungui, E, Pinnagoda, K, Hubbell, JA & Frey, P 2015, 'Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System', Tissue Engineering - Part A, vol. 21, no. 17-18, pp. 2334-2345. https://doi.org/10.1089/ten.tea.2015.0048
Vardar E, Engelhardt EM, Larsson HM, Mouloungui E, Pinnagoda K, Hubbell JA et al. Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System. Tissue Engineering - Part A. 2015 Sep 1;21(17-18):2334-2345. https://doi.org/10.1089/ten.tea.2015.0048
Vardar, Elif ; Engelhardt, Eva Maria ; Larsson, Hans M. ; Mouloungui, Elodie ; Pinnagoda, Kalitha ; Hubbell, Jeffrey A. ; Frey, Peter. / Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System. In: Tissue Engineering - Part A. 2015 ; Vol. 21, No. 17-18. pp. 2334-2345.
@article{25e68a29fbd54dc1b4c9a17d0fc63abc,
title = "Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System",
abstract = "Ureteral replacement by tissue engineering might become necessary following tissue loss after excessive ureteral trauma, after retroperitoneal cancer, or even after failed reconstructive surgery. This need has driven innovation in the design of novel scaffolds and specific cell culture techniques for urinary tract reconstruction. In this study, compressed tubular collagen scaffolds were evaluated, addressing the physical and biological characterization of acellular and cellular collagen tubes in a new flow bioreactor system, imitating the physiological pressure, peristalsis, and flow conditions of the human ureter. Collagen tubes, containing primary human smooth muscle and urothelial cells, were evaluated regarding their change in gene and protein expression under dynamic culture conditions. A maximum intraluminal pressure of 22.43±0.2cmH2O was observed in acellular tubes, resulting in a mean wall shear stress of 4 dynes/cm2 in the tubular constructs. Dynamic conditions directed the differentiation of both cell types into their mature forms. This was confirmed by their gene expression of smooth muscle alpha-actin, smoothelin, collagen type I and III, elastin, laminin type 1 and 5, cytokeratin 8, and uroplakin 2. In addition, smooth muscle cell alignment predominantly perpendicular to the flow direction was observed, comparable to the cell orientation in native ureteral tissue. These results revealed that coculturing human smooth muscle and urothelial cells in compressed collagen tubes under human ureteral flow-mimicking conditions could lead to cell-engineered biomaterials that might ultimately be translated into clinical applications.",
author = "Elif Vardar and Engelhardt, {Eva Maria} and Larsson, {Hans M.} and Elodie Mouloungui and Kalitha Pinnagoda and Hubbell, {Jeffrey A.} and Peter Frey",
year = "2015",
month = "9",
day = "1",
doi = "10.1089/ten.tea.2015.0048",
language = "English (US)",
volume = "21",
pages = "2334--2345",
journal = "Tissue Engineering - Part A",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "17-18",

}

TY - JOUR

T1 - Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System

AU - Vardar, Elif

AU - Engelhardt, Eva Maria

AU - Larsson, Hans M.

AU - Mouloungui, Elodie

AU - Pinnagoda, Kalitha

AU - Hubbell, Jeffrey A.

AU - Frey, Peter

PY - 2015/9/1

Y1 - 2015/9/1

N2 - Ureteral replacement by tissue engineering might become necessary following tissue loss after excessive ureteral trauma, after retroperitoneal cancer, or even after failed reconstructive surgery. This need has driven innovation in the design of novel scaffolds and specific cell culture techniques for urinary tract reconstruction. In this study, compressed tubular collagen scaffolds were evaluated, addressing the physical and biological characterization of acellular and cellular collagen tubes in a new flow bioreactor system, imitating the physiological pressure, peristalsis, and flow conditions of the human ureter. Collagen tubes, containing primary human smooth muscle and urothelial cells, were evaluated regarding their change in gene and protein expression under dynamic culture conditions. A maximum intraluminal pressure of 22.43±0.2cmH2O was observed in acellular tubes, resulting in a mean wall shear stress of 4 dynes/cm2 in the tubular constructs. Dynamic conditions directed the differentiation of both cell types into their mature forms. This was confirmed by their gene expression of smooth muscle alpha-actin, smoothelin, collagen type I and III, elastin, laminin type 1 and 5, cytokeratin 8, and uroplakin 2. In addition, smooth muscle cell alignment predominantly perpendicular to the flow direction was observed, comparable to the cell orientation in native ureteral tissue. These results revealed that coculturing human smooth muscle and urothelial cells in compressed collagen tubes under human ureteral flow-mimicking conditions could lead to cell-engineered biomaterials that might ultimately be translated into clinical applications.

AB - Ureteral replacement by tissue engineering might become necessary following tissue loss after excessive ureteral trauma, after retroperitoneal cancer, or even after failed reconstructive surgery. This need has driven innovation in the design of novel scaffolds and specific cell culture techniques for urinary tract reconstruction. In this study, compressed tubular collagen scaffolds were evaluated, addressing the physical and biological characterization of acellular and cellular collagen tubes in a new flow bioreactor system, imitating the physiological pressure, peristalsis, and flow conditions of the human ureter. Collagen tubes, containing primary human smooth muscle and urothelial cells, were evaluated regarding their change in gene and protein expression under dynamic culture conditions. A maximum intraluminal pressure of 22.43±0.2cmH2O was observed in acellular tubes, resulting in a mean wall shear stress of 4 dynes/cm2 in the tubular constructs. Dynamic conditions directed the differentiation of both cell types into their mature forms. This was confirmed by their gene expression of smooth muscle alpha-actin, smoothelin, collagen type I and III, elastin, laminin type 1 and 5, cytokeratin 8, and uroplakin 2. In addition, smooth muscle cell alignment predominantly perpendicular to the flow direction was observed, comparable to the cell orientation in native ureteral tissue. These results revealed that coculturing human smooth muscle and urothelial cells in compressed collagen tubes under human ureteral flow-mimicking conditions could lead to cell-engineered biomaterials that might ultimately be translated into clinical applications.

UR - http://www.scopus.com/inward/record.url?scp=84940651066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940651066&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2015.0048

DO - 10.1089/ten.tea.2015.0048

M3 - Article

AN - SCOPUS:84940651066

VL - 21

SP - 2334

EP - 2345

JO - Tissue Engineering - Part A

JF - Tissue Engineering - Part A

SN - 1937-3341

IS - 17-18

ER -