Tsc1+ and tsc2+ Regulate Arginine Uptake and Metabolism in Schizosaccharomyces pombe

Marjon Van Slegtenhorst, Erikka Carr, Radka Stoyanova, Warren D. Kruger, Elizabeth Petri Henske

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Mutations in either TSC1 or TSC2 cause tuberous sclerosis complex, an autosomal dominant disorder characterized by seizures, mental retardation, and benign tumors of the skin, brain, heart, and kidneys. Homologs for the TSC1 and TSC2 genes have been identified in mouse, rat, Fugu, Drosophila, and in the yeast Schizosaccharomyces pombe. Here we show that S. pombe lacking tsc1 + or tsc2+ have similar phenotypes including decreased arginine uptake, decreased expression of three amino acid permeases, and low intracellular levels of four members of the arginine biosynthesis pathway. Recently, the small GTPase Rheb was identified as a target of the GTPase-activating domain of tuberin in mammalian cells and in Drosophila. We show that the defect in arginine uptake in cells lacking tsc2+ is rescued by the expression of a dominant negative form of rhb1+, the Rheb homolog in S. pombe, but not by expressing wild-type rhb1+. Expression of the tsc2+ gene with a patient-derived mutation within the GAP domain did not rescue the arginine uptake defect in tsc2+ mutant yeast. Taken together, these findings support a model in which arginine uptake is regulated through tsc1+, tsc2+, and rhb1 + in S. pombe and also suggest a role for the Tsc1 and Tsc2 proteins in amino acid biosynthesis and sensing.

Original languageEnglish
Pages (from-to)12706-12713
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number13
DOIs
StatePublished - Mar 26 2004
Externally publishedYes

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Schizosaccharomyces
Metabolism
Arginine
Biosynthesis
Yeast
Drosophila
Genes
Yeasts
Takifugu
Amino Acid Transport Systems
Defects
Mutation
Tuberous Sclerosis
Monomeric GTP-Binding Proteins
GTP Phosphohydrolases
Brain Neoplasms
Intellectual Disability
Rats
Tumors
Epilepsy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tsc1+ and tsc2+ Regulate Arginine Uptake and Metabolism in Schizosaccharomyces pombe. / Van Slegtenhorst, Marjon; Carr, Erikka; Stoyanova, Radka; Kruger, Warren D.; Henske, Elizabeth Petri.

In: Journal of Biological Chemistry, Vol. 279, No. 13, 26.03.2004, p. 12706-12713.

Research output: Contribution to journalArticle

Van Slegtenhorst, Marjon ; Carr, Erikka ; Stoyanova, Radka ; Kruger, Warren D. ; Henske, Elizabeth Petri. / Tsc1+ and tsc2+ Regulate Arginine Uptake and Metabolism in Schizosaccharomyces pombe. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 13. pp. 12706-12713.
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