Tropomyosin in peripheral ruffles of cultured rat kidney cells

R. H. Warren, E. Gordon, R. Azarnia

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Tropomyosin distribution has been studied in two normal lines and one transformed line of rat kidney cells during the early phases of substrate attachment and growth. One non-motile normal line, which spreads rapidly after attachment, immediately begins to assemble prominent stress fibers that contain tropomyosin. It displays small peripheral ruffles that are not noticeably stained with anti-tropomyosin. The other normal line is motile and produces large ruffles that are brightly stained with anti-tropomyosin. Large numbers of tropomyosin-positive stress fibers assemble only after the cells stop moving and lose the peripheral ruffles. The transformed line does not assemble stress fibers but does contain large numbers of actin filament bundles in ruffles on the cell surface that are stained with anti-tropomyosin. These observations indicate that cytoskeletal tropomyosin is not restricted in distribution to stress fibers, and may undergo re-organization along with actin during the transition from motile to non-motile behavior.

Original languageEnglish (US)
Pages (from-to)245-253
Number of pages9
JournalEuropean Journal of Cell Biology
Volume38
Issue number2
StatePublished - Dec 1 1985

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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    Warren, R. H., Gordon, E., & Azarnia, R. (1985). Tropomyosin in peripheral ruffles of cultured rat kidney cells. European Journal of Cell Biology, 38(2), 245-253.