tRNA nucleotidyltransferase is not essential for Escherichia coli viability.

L. Zhu, Murray P Deutscher

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The role of tRNA nucleotidyltransferase in Escherichia coli has been uncertain because all tRNA genes studied in this organism already encode the -C-C-A sequence. Examination of a cca mutant, originally thought to contain 1-2% enzyme activity, indicated that it actually produces an inactive fragment of 40 kd compared to 47 kd for the wild-type enzyme due to a nonsense mutation in its cca gene. To confirm that the residual activity in extracts of this strain is due to another enzyme, and that tRNA nucleotidyltransferase is non-essential, we have interrupted the cca gene in vitro, and transferred this mutant gene to a variety of strains. In all cases mutant strains are viable, although as much as 15% of the tRNA population contains defective 3' termini, and no tRNA nucleotidyltransferase is detectable. Mutant strains grow slowly, but can be restored to more normal growth by a relA mutation or by a decrease in RNase T activity. In the latter case the amount of defective tRNA decreases dramatically. These findings indicate that tRNA nucleotidyltransferase is not essential for E. coli viability, and therefore, that all essential tRNA genes in this organism encode the -C-C-A sequence.

Original languageEnglish
Pages (from-to)2473-2477
Number of pages5
JournalEMBO Journal
Volume6
Issue number8
StatePublished - Aug 1 1987
Externally publishedYes

Fingerprint

Transfer RNA
Escherichia coli
Genes
Enzymes
Nonsense Codon
Essential Genes
Enzyme activity
Mutation
tRNA nucleotidyltransferase
Growth
Population

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

tRNA nucleotidyltransferase is not essential for Escherichia coli viability. / Zhu, L.; Deutscher, Murray P.

In: EMBO Journal, Vol. 6, No. 8, 01.08.1987, p. 2473-2477.

Research output: Contribution to journalArticle

Zhu, L & Deutscher, MP 1987, 'tRNA nucleotidyltransferase is not essential for Escherichia coli viability.', EMBO Journal, vol. 6, no. 8, pp. 2473-2477.
Zhu, L. ; Deutscher, Murray P. / tRNA nucleotidyltransferase is not essential for Escherichia coli viability. In: EMBO Journal. 1987 ; Vol. 6, No. 8. pp. 2473-2477.
@article{36a716f004c5439ab6ff9253764a2110,
title = "tRNA nucleotidyltransferase is not essential for Escherichia coli viability.",
abstract = "The role of tRNA nucleotidyltransferase in Escherichia coli has been uncertain because all tRNA genes studied in this organism already encode the -C-C-A sequence. Examination of a cca mutant, originally thought to contain 1-2{\%} enzyme activity, indicated that it actually produces an inactive fragment of 40 kd compared to 47 kd for the wild-type enzyme due to a nonsense mutation in its cca gene. To confirm that the residual activity in extracts of this strain is due to another enzyme, and that tRNA nucleotidyltransferase is non-essential, we have interrupted the cca gene in vitro, and transferred this mutant gene to a variety of strains. In all cases mutant strains are viable, although as much as 15{\%} of the tRNA population contains defective 3' termini, and no tRNA nucleotidyltransferase is detectable. Mutant strains grow slowly, but can be restored to more normal growth by a relA mutation or by a decrease in RNase T activity. In the latter case the amount of defective tRNA decreases dramatically. These findings indicate that tRNA nucleotidyltransferase is not essential for E. coli viability, and therefore, that all essential tRNA genes in this organism encode the -C-C-A sequence.",
author = "L. Zhu and Deutscher, {Murray P}",
year = "1987",
month = "8",
day = "1",
language = "English",
volume = "6",
pages = "2473--2477",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - tRNA nucleotidyltransferase is not essential for Escherichia coli viability.

AU - Zhu, L.

AU - Deutscher, Murray P

PY - 1987/8/1

Y1 - 1987/8/1

N2 - The role of tRNA nucleotidyltransferase in Escherichia coli has been uncertain because all tRNA genes studied in this organism already encode the -C-C-A sequence. Examination of a cca mutant, originally thought to contain 1-2% enzyme activity, indicated that it actually produces an inactive fragment of 40 kd compared to 47 kd for the wild-type enzyme due to a nonsense mutation in its cca gene. To confirm that the residual activity in extracts of this strain is due to another enzyme, and that tRNA nucleotidyltransferase is non-essential, we have interrupted the cca gene in vitro, and transferred this mutant gene to a variety of strains. In all cases mutant strains are viable, although as much as 15% of the tRNA population contains defective 3' termini, and no tRNA nucleotidyltransferase is detectable. Mutant strains grow slowly, but can be restored to more normal growth by a relA mutation or by a decrease in RNase T activity. In the latter case the amount of defective tRNA decreases dramatically. These findings indicate that tRNA nucleotidyltransferase is not essential for E. coli viability, and therefore, that all essential tRNA genes in this organism encode the -C-C-A sequence.

AB - The role of tRNA nucleotidyltransferase in Escherichia coli has been uncertain because all tRNA genes studied in this organism already encode the -C-C-A sequence. Examination of a cca mutant, originally thought to contain 1-2% enzyme activity, indicated that it actually produces an inactive fragment of 40 kd compared to 47 kd for the wild-type enzyme due to a nonsense mutation in its cca gene. To confirm that the residual activity in extracts of this strain is due to another enzyme, and that tRNA nucleotidyltransferase is non-essential, we have interrupted the cca gene in vitro, and transferred this mutant gene to a variety of strains. In all cases mutant strains are viable, although as much as 15% of the tRNA population contains defective 3' termini, and no tRNA nucleotidyltransferase is detectable. Mutant strains grow slowly, but can be restored to more normal growth by a relA mutation or by a decrease in RNase T activity. In the latter case the amount of defective tRNA decreases dramatically. These findings indicate that tRNA nucleotidyltransferase is not essential for E. coli viability, and therefore, that all essential tRNA genes in this organism encode the -C-C-A sequence.

UR - http://www.scopus.com/inward/record.url?scp=0023394389&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023394389&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 2473

EP - 2477

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 8

ER -