Abstract
Although CNS axons have the capacity to regenerate after spinal cord injury when provided with a permissive substrate, the lack of appropriate synaptic target sites for regenerating fibers may limit restoration of spinal circuitry. Studies in our laboratory are focused on utilizing neural stem cells to provide new synaptic target sites for regenerating spinal axons following injury. As an initial step, rat neural precursor cells genetically engineered to overexpress the tyrosine kinase C (trkC) neurotrophin receptor were transplanted into the intact rat spinal cord to evaluate their survival and differentiation. Cells were either pretreated in vitro prior to transplantation with trkC ligand neurotrophin-3 (NT-3) to initiate differentiation or exposed to NT-3 in vivo following transplantation via gelfoam or Oxycel
| Original language | English |
|---|---|
| Pages (from-to) | 297-307 |
| Number of pages | 11 |
| Journal | Cell Transplantation |
| Volume | 11 |
| Issue number | 3 |
| State | Published - Jul 2 2002 |
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Keywords
- Neural precursor cells
- Neural transplantation
- Neurotrophin-3
- Spinal cord
- Stem cells
ASJC Scopus subject areas
- Cell Biology
- Transplantation
Cite this
TrkC overexpression enhances survival and migration of neural stem cell transplants in the rat spinal cord. / Castellanos, Daniel A.; Tsoulfas, Pantelis; Frydel, Beata R.; Gajavelli, Shyam; Bes, Jean Claude; Sagen, Jacqueline.
In: Cell Transplantation, Vol. 11, No. 3, 02.07.2002, p. 297-307.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - TrkC overexpression enhances survival and migration of neural stem cell transplants in the rat spinal cord
AU - Castellanos, Daniel A.
AU - Tsoulfas, Pantelis
AU - Frydel, Beata R.
AU - Gajavelli, Shyam
AU - Bes, Jean Claude
AU - Sagen, Jacqueline
PY - 2002/7/2
Y1 - 2002/7/2
N2 - Although CNS axons have the capacity to regenerate after spinal cord injury when provided with a permissive substrate, the lack of appropriate synaptic target sites for regenerating fibers may limit restoration of spinal circuitry. Studies in our laboratory are focused on utilizing neural stem cells to provide new synaptic target sites for regenerating spinal axons following injury. As an initial step, rat neural precursor cells genetically engineered to overexpress the tyrosine kinase C (trkC) neurotrophin receptor were transplanted into the intact rat spinal cord to evaluate their survival and differentiation. Cells were either pretreated in vitro prior to transplantation with trkC ligand neurotrophin-3 (NT-3) to initiate differentiation or exposed to NT-3 in vivo following transplantation via gelfoam or Oxycel
AB - Although CNS axons have the capacity to regenerate after spinal cord injury when provided with a permissive substrate, the lack of appropriate synaptic target sites for regenerating fibers may limit restoration of spinal circuitry. Studies in our laboratory are focused on utilizing neural stem cells to provide new synaptic target sites for regenerating spinal axons following injury. As an initial step, rat neural precursor cells genetically engineered to overexpress the tyrosine kinase C (trkC) neurotrophin receptor were transplanted into the intact rat spinal cord to evaluate their survival and differentiation. Cells were either pretreated in vitro prior to transplantation with trkC ligand neurotrophin-3 (NT-3) to initiate differentiation or exposed to NT-3 in vivo following transplantation via gelfoam or Oxycel
KW - Neural precursor cells
KW - Neural transplantation
KW - Neurotrophin-3
KW - Spinal cord
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=0035998731&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035998731&partnerID=8YFLogxK
M3 - Article
VL - 11
SP - 297
EP - 307
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 3
ER -