TrkC overexpression enhances survival and migration of neural stem cell transplants in the rat spinal cord

Daniel A. Castellanos, Pantelis Tsoulfas, Beata R. Frydel, Shyam Gajavelli, Jean Claude Bes, Jacqueline Sagen

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Although CNS axons have the capacity to regenerate after spinal cord injury when provided with a permissive substrate, the lack of appropriate synaptic target sites for regenerating fibers may limit restoration of spinal circuitry. Studies in our laboratory are focused on utilizing neural stem cells to provide new synaptic target sites for regenerating spinal axons following injury. As an initial step, rat neural precursor cells genetically engineered to overexpress the tyrosine kinase C (trkC) neurotrophin receptor were transplanted into the intact rat spinal cord to evaluate their survival and differentiation. Cells were either pretreated in vitro prior to transplantation with trkC ligand neurotrophin-3 (NT-3) to initiate differentiation or exposed to NT-3 in vivo following transplantation via gelfoam or Oxycel

Original languageEnglish (US)
Pages (from-to)297-307
Number of pages11
JournalCell Transplantation
Volume11
Issue number3
StatePublished - Jul 2 2002

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Keywords

  • Neural precursor cells
  • Neural transplantation
  • Neurotrophin-3
  • Spinal cord
  • Stem cells

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

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