Triple-negative breast cancers express receptors for luteinizing hormone-releasing hormone (LHRH) and respond to LHRH antagonist Cetrorelix with growth inhibition

Stefan Buchholz, Stephan Seitz, Andrew V Schally, Jörg B. Engel, Ferenc G. Rick, Luca Szalontay, Florian Hohla, Awtar Krishan, Andrea Papadia, Timo Gaiser, Gero Brockhoff, Olaf Ortmann, Klaus Diedrich, Frank Köster

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The aim of the present study was to evaluate the expression of receptors for luteinizing hormone-releasing hormone (LHRH) in human specimens of triple-negative breast cancers (TNBC). In addition, we used in vitro and in vivo models of TNBC to investigate if these receptors are suitable targets for the treatment with the LHRH antagonist Cetrorelix. Receptors for LHRH were expressed in all tumor samples and in the TNBC cell lines HCC1806 and HCC1937. The proliferation of both TNBC cell lines was significantly inhibited in vitro by 1 μM Cetrorelix. Injections of 3 mg Cetrorelix on day 1 and 21 resulted in a significant growth inhibition of HCC1806 tumors xenografted into nude mice. Tumors of mice treated with Cetrorelix expressed less mRNA for EGFR and HER3 receptors than untreated tumors. After treatment of cells with Cetrorelix a flow cytometric analysis of the cell cycle revealed a decrease in S-phase. Given the low toxicity and clinical availability of Cetrorelix, this peptide antagonist should be considered for phase II studies in patients with advanced TNBC.

Original languageEnglish
Pages (from-to)789-796
Number of pages8
JournalInternational Journal of Oncology
Volume35
Issue number4
DOIs
StatePublished - Oct 1 2009

Fingerprint

Triple Negative Breast Neoplasms
LHRH Receptors
Hormone Antagonists
Gonadotropin-Releasing Hormone
Growth
Neoplasms
Cell Line
S Phase
Nude Mice
cetrorelix
Cell Cycle
Messenger RNA
Peptides
Injections
Therapeutics

Keywords

  • Cell cycle analysis
  • LHRH antagonist
  • LHRH receptor
  • Triple-negative breast cancer
  • Xenografted mouse model

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Triple-negative breast cancers express receptors for luteinizing hormone-releasing hormone (LHRH) and respond to LHRH antagonist Cetrorelix with growth inhibition. / Buchholz, Stefan; Seitz, Stephan; Schally, Andrew V; Engel, Jörg B.; Rick, Ferenc G.; Szalontay, Luca; Hohla, Florian; Krishan, Awtar; Papadia, Andrea; Gaiser, Timo; Brockhoff, Gero; Ortmann, Olaf; Diedrich, Klaus; Köster, Frank.

In: International Journal of Oncology, Vol. 35, No. 4, 01.10.2009, p. 789-796.

Research output: Contribution to journalArticle

Buchholz, S, Seitz, S, Schally, AV, Engel, JB, Rick, FG, Szalontay, L, Hohla, F, Krishan, A, Papadia, A, Gaiser, T, Brockhoff, G, Ortmann, O, Diedrich, K & Köster, F 2009, 'Triple-negative breast cancers express receptors for luteinizing hormone-releasing hormone (LHRH) and respond to LHRH antagonist Cetrorelix with growth inhibition', International Journal of Oncology, vol. 35, no. 4, pp. 789-796. https://doi.org/10.3892/ijo-00000391
Buchholz, Stefan ; Seitz, Stephan ; Schally, Andrew V ; Engel, Jörg B. ; Rick, Ferenc G. ; Szalontay, Luca ; Hohla, Florian ; Krishan, Awtar ; Papadia, Andrea ; Gaiser, Timo ; Brockhoff, Gero ; Ortmann, Olaf ; Diedrich, Klaus ; Köster, Frank. / Triple-negative breast cancers express receptors for luteinizing hormone-releasing hormone (LHRH) and respond to LHRH antagonist Cetrorelix with growth inhibition. In: International Journal of Oncology. 2009 ; Vol. 35, No. 4. pp. 789-796.
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