Trinitrophenyl modification of H-2k and H-2b spleen cells results in enhanced serological detection of Kk-like determinants

R. B. Levy, S. K. Dower, G. M. Shearer, D. M. Segal

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Several anti-H-2K(k) but not anti-H-2D(d) monoclonal antibodies (mAb) exhibited enhanced binding to B10.A murine spleen cells after modification of the cells with trinitrobenzene sulfonate (TNBS). The number of antibody molecules bound to TNP-modified B10.A spleen cells increased by a factor of two or more. The same anti-2K(k) mAb that exhibited enhanced binding to modified B10.A cells did not bind to unmodified C57BL/10 spleen cells, as expected, but did bind to TNP-modified C57BL/10 spleen cells. This TNP-dependent binding was not a result of cross-reactions with cell surface TNP groups nor with Fc receptors. TNP modification of a variant cell line that does not express class I H-2 products did not result in enhanced binding by these mAb. These findings can account for preferential recognition of TNP-K(k) by B10.A and B10.BR CTL, and also for cross-reactive lysis by C57BL/10 CTL stimulated by C57BL/10-TNP against unmodified H-2K(k) targets.

Original languageEnglish (US)
Pages (from-to)1464-1472
Number of pages9
JournalJournal of Experimental Medicine
Issue number5
StatePublished - May 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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