Trinitrophenyl modification of H-2(k) and H-2(b) spleen cells results in enhanced serological detection of K(k)-like determinants

Robert B Levy, S. K. Dower, G. M. Shearer, D. M. Segal

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Abstract

Several anti-H-2K(k) but not anti-H-2D(d) monoclonal antibodies (mAb) exhibited enhanced binding to B10.A murine spleen cells after modification of the cells with trinitrobenzene sulfonate (TNBS). The number of antibody molecules bound to TNP-modified B10.A spleen cells increased by a factor of two or more. The same anti-2K(k) mAb that exhibited enhanced binding to modified B10.A cells did not bind to unmodified C57BL/10 spleen cells, as expected, but did bind to TNP-modified C57BL/10 spleen cells. This TNP-dependent binding was not a result of cross-reactions with cell surface TNP groups nor with Fc receptors. TNP modification of a variant cell line that does not express class I H-2 products did not result in enhanced binding by these mAb. These findings can account for preferential recognition of TNP-K(k) by B10.A and B10.BR CTL, and also for cross-reactive lysis by C57BL/10 CTL stimulated by C57BL/10-TNP against unmodified H-2K(k) targets.

Original languageEnglish
Pages (from-to)1464-1472
Number of pages9
JournalJournal of Experimental Medicine
Volume159
Issue number5
StatePublished - Jan 1 1984
Externally publishedYes

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Spleen
Monoclonal Antibodies
Trinitrobenzenesulfonic Acid
Fc Receptors
Cross Reactions
Cell Line
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Trinitrophenyl modification of H-2(k) and H-2(b) spleen cells results in enhanced serological detection of K(k)-like determinants. / Levy, Robert B; Dower, S. K.; Shearer, G. M.; Segal, D. M.

In: Journal of Experimental Medicine, Vol. 159, No. 5, 01.01.1984, p. 1464-1472.

Research output: Contribution to journalArticle

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abstract = "Several anti-H-2K(k) but not anti-H-2D(d) monoclonal antibodies (mAb) exhibited enhanced binding to B10.A murine spleen cells after modification of the cells with trinitrobenzene sulfonate (TNBS). The number of antibody molecules bound to TNP-modified B10.A spleen cells increased by a factor of two or more. The same anti-2K(k) mAb that exhibited enhanced binding to modified B10.A cells did not bind to unmodified C57BL/10 spleen cells, as expected, but did bind to TNP-modified C57BL/10 spleen cells. This TNP-dependent binding was not a result of cross-reactions with cell surface TNP groups nor with Fc receptors. TNP modification of a variant cell line that does not express class I H-2 products did not result in enhanced binding by these mAb. These findings can account for preferential recognition of TNP-K(k) by B10.A and B10.BR CTL, and also for cross-reactive lysis by C57BL/10 CTL stimulated by C57BL/10-TNP against unmodified H-2K(k) targets.",
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