Trifluorothymidine and 5-fluorouracil: Antiproliferative activity in tissue culture

A. H. Rivera, A. S. Hajek, F. Fantes, K. S. Mallick, R. K. Parrish

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The antiproliferative activity of trifluorothymidine (F3TDR) and 5-fluorouracil (5-FU), two cell-cycle-phase-specific antimetabolites, was compared in a tissue culture model of human scleral fibroblasts and rabbit corneal epithelial cells. The mean concentrations required to inhibit human scleral fibroblast proliferation to 50% of the control rate (ID50) after 5 days of exposure were 8.50 μg/mL for F3TDR and 0.3 μg/mL for 5-FU. The corresponding ID50 levels for rabbit corneal epithelial cells were 0.24 μg/mL and 0.42 μg/mL. The ID50 of F3TDR was significantly higher than that of 5-FU in human scleral fibroblasts (p <0.001) but not in rabbit corneal epithelial cells. F3TDR appears to have no clinical advantage over 5-FU in minimizing corneal epithelial toxicity when given to prevent fibroblast proliferation after glaucoma filtering surgery.

Original languageEnglish (US)
Pages (from-to)13-16
Number of pages4
JournalCanadian Journal of Ophthalmology
Volume22
Issue number1
StatePublished - Sep 10 1987
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology

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