Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro

Jose Guzman, Jose Ruiz, Adrien Eshraghi, Marek Polak, Carolyn Garnham, Thomas Balkany, Thomas Van De Water

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Conclusion. Triamcinolone acetonide crystalline suspension (e.g. Volon A®) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage. Objectives. To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants. Materials and methods. Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p < /0.05. Results. Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.

Original languageEnglish (US)
Pages (from-to)685-690
Number of pages6
JournalActa Oto-Laryngologica
Volume126
Issue number7
DOIs
StatePublished - Jul 1 2006

Keywords

  • Corticosteroids
  • Inner hair cells
  • Organ culture
  • Organ of Corti
  • Otoprotection
  • Ototoxicity
  • Outer hair cells
  • Oxidative stress

ASJC Scopus subject areas

  • Otorhinolaryngology

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