Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro

Jose Guzman, Jose Ruiz, Adrien Eshraghi, Marek Polak, Carolyn Garnham, Thomas Balkany, Thomas R Van De Water

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Conclusion. Triamcinolone acetonide crystalline suspension (e.g. Volon A®) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage. Objectives. To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants. Materials and methods. Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p < /0.05. Results. Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.

Original languageEnglish
Pages (from-to)685-690
Number of pages6
JournalActa Oto-Laryngologica
Volume126
Issue number7
DOIs
StatePublished - Jul 1 2006

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Auditory Hair Cells
Organ of Corti
Triamcinolone Acetonide
Cell Count
Phalloidine
Triamcinolone
Cytoprotection
Fluorescein-5-isothiocyanate
Microscopy
Suspensions
Adrenal Cortex Hormones
Oxidative Stress
4-hydroxy-2-nonenal
In Vitro Techniques

Keywords

  • Corticosteroids
  • Inner hair cells
  • Organ culture
  • Organ of Corti
  • Otoprotection
  • Ototoxicity
  • Outer hair cells
  • Oxidative stress

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro. / Guzman, Jose; Ruiz, Jose; Eshraghi, Adrien; Polak, Marek; Garnham, Carolyn; Balkany, Thomas; Van De Water, Thomas R.

In: Acta Oto-Laryngologica, Vol. 126, No. 7, 01.07.2006, p. 685-690.

Research output: Contribution to journalArticle

Guzman, Jose ; Ruiz, Jose ; Eshraghi, Adrien ; Polak, Marek ; Garnham, Carolyn ; Balkany, Thomas ; Van De Water, Thomas R. / Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro. In: Acta Oto-Laryngologica. 2006 ; Vol. 126, No. 7. pp. 685-690.
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