Abstract
Conclusion. Triamcinolone acetonide crystalline suspension (e.g. Volon A®) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage. Objectives. To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants. Materials and methods. Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p < /0.05. Results. Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.
Original language | English (US) |
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Pages (from-to) | 685-690 |
Number of pages | 6 |
Journal | Acta Oto-Laryngologica |
Volume | 126 |
Issue number | 7 |
DOIs | |
State | Published - Jul 1 2006 |
Keywords
- Corticosteroids
- Inner hair cells
- Organ culture
- Organ of Corti
- Otoprotection
- Ototoxicity
- Outer hair cells
- Oxidative stress
ASJC Scopus subject areas
- Otorhinolaryngology