Abstract
Conclusion. Triamcinolone acetonide crystalline suspension (e.g. Volon A®) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage. Objectives. To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants. Materials and methods. Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p < /0.05. Results. Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.
| Original language | English |
|---|---|
| Pages (from-to) | 685-690 |
| Number of pages | 6 |
| Journal | Acta Oto-Laryngologica |
| Volume | 126 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2006 |
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Keywords
- Corticosteroids
- Inner hair cells
- Organ culture
- Organ of Corti
- Otoprotection
- Ototoxicity
- Outer hair cells
- Oxidative stress
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro. / Guzman, Jose; Ruiz, Jose; Eshraghi, Adrien; Polak, Marek; Garnham, Carolyn; Balkany, Thomas; Van De Water, Thomas R.
In: Acta Oto-Laryngologica, Vol. 126, No. 7, 01.07.2006, p. 685-690.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Triamcinolone acetonide protects auditory hair cells from 4-hydroxy-2,3-nonenal (HNE) ototoxicity in vitro
AU - Guzman, Jose
AU - Ruiz, Jose
AU - Eshraghi, Adrien
AU - Polak, Marek
AU - Garnham, Carolyn
AU - Balkany, Thomas
AU - Van De Water, Thomas R
PY - 2006/7/1
Y1 - 2006/7/1
N2 - Conclusion. Triamcinolone acetonide crystalline suspension (e.g. Volon A®) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage. Objectives. To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants. Materials and methods. Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p < /0.05. Results. Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.
AB - Conclusion. Triamcinolone acetonide crystalline suspension (e.g. Volon A®) was not ototoxic to the auditory hair cells present within organ of Corti explants and protected them from an ototoxic molecule, i.e. 4-hydroxy-2,3-nonenal (HNE), that is produced within the organ of Corti as a result of oxidative stress-induced damage. Objectives. To test the corticosteroid, triamcinolone acetonide, for ototoxicity and otoprotective capacity in organ of Corti explants. Materials and methods. Organ of Corti explants excised from 4-day-old rats were the test system, HNE was the ototoxin challenge. Hair cell integrity counts were performed with fluorescent microscopy on fixed explants stained with FITC-labeled phalloidin. Statistical significance was set at p < /0.05. Results. Triamcinolone acetonide did not affect hair cell integrity in the organ of Corti explants and it provided a high level of protection of hair cells against the ototoxic effects of a damaging level of HNE as determined by hair cell density counts.
KW - Corticosteroids
KW - Inner hair cells
KW - Organ culture
KW - Organ of Corti
KW - Otoprotection
KW - Ototoxicity
KW - Outer hair cells
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=33745698846&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745698846&partnerID=8YFLogxK
U2 - 10.1080/00016480500492018
DO - 10.1080/00016480500492018
M3 - Article
VL - 126
SP - 685
EP - 690
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
SN - 0001-6489
IS - 7
ER -