Treatment with analgesics after mouse sciatic nerve injury does not alter expression of wound healingassociated genes

Matt C. Danzi, Dario Motti, Donna L. Avison, John L. Bixby, Vance P. Lemmon

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Animal models of sciatic nerve injury are commonly used to study neuropathic pain as well as axon regeneration. Administration of post-surgical analgesics is an important consideration for animal welfare, but the actions of the analgesic must not interfere with the scientific goals of the experiment. In this study, we show that treatment with either buprenorphine or acetaminophen following a bilateral sciatic nerve crush surgery does not alter the expression in dorsal root ganglion (DRG) sensory neurons of a panel of genes associated with wound healing. These findings indicate that the post-operative use of buprenorphine or acetaminophen at doses commonly suggested by Institutional Animal Care and Use Committees does not change the intrinsic gene expression response of DRG neurons to a sciatic nerve crush injury, for many wound healing-associated genes. Therefore, administration of post-operative analgesics may not confound the results of transcriptomic studies employing this injury model.

Original languageEnglish (US)
Pages (from-to)144-149
Number of pages6
JournalNeural Regeneration Research
Volume11
Issue number1
DOIs
StatePublished - Jan 2016

Keywords

  • Acetaminophen
  • Analgesics
  • Axon
  • Buprenorphine
  • Dorsal root ganglia
  • Gene expression
  • Peripheral nerve injuries
  • Regeneration
  • Sciatic nerve
  • Wound healing

ASJC Scopus subject areas

  • Developmental Neuroscience

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